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Figure 5 | Retrovirology

Figure 5

From: Inhibition of HIV-1 gene expression by Ciclopirox and Deferiprone, drugs that prevent hypusination of eukaryotic initiation factor 5A

Figure 5

Sequence requirements for the drug sensitivity of the HIV molecular clone. A. Schematic of constructs expressing firefly luciferase from the CMV promoter (construct I, pCMV-FF) or the HIV promoter. Constructs III, IV and V were generated by deleting sequences from pNL4-3-LucE- (construct II). Construct VI was made by replacing the 3'LTR in construct V with the SV40 poly(A) sequence from pGL2TAR. Construct VII is a chimera of pGL2TAR and construct VI. B. CPX and DEF sensitivity of the constructs. Firefly luciferase expression from each construct was normalized to Renilla luciferase expression from pCMV-Ren as in Fig. 3, and presented as a percentage of the control ratio obtained in the absence of drugs.

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Retrovirology

ISSN: 1742-4690

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