Figure 4From: Proviral integrations and expression of endogenous Avian leucosis virus during long term selection for high and low body weight in two chicken linesDetermination of the number of proviral integrations in different chicken populations. A. Standard curve based on diluted plasmid with a PCR product. B. Relative copy numbers of env gene were examined by qPCR with primer d* and the numbers of integration per haploid genome were determined using the standard curve shown in A. Horizontal bars represent mean values of integration number for each population. One-way ANOVA together with Newman-Keuls test as a post-hoc analysis was utilized. Mean ± SEM: F9 = 15.42 ± 0.31, H41 = 11.01 ± 0.83, L41 = 14.34 ± 1.01, H45 = 8.87 ± 0.42, L45 = 13.73 ± 0.51, WL = 5.56 ± 0.46 and RJ = 3.59 ± 0.56. N = 82 in F9 birds and n = 10 in the other populations. F9, generation 9 of the advanced intercross line; H41 and L41, generation 41 of the HWS and LWS lines, respectively; H45 and L45, generation 45 of the parental lines; WL, White Leghorn and RJF, Red Jungle Fowl.Back to article page