Figure 1From: Proviral integrations and expression of endogenous Avian leucosis virus during long term selection for high and low body weight in two chicken linesSchematic ALV genome with PCR amplicons and SNPs. A. Schematic time-line with parental generations and crosses. Generations in boxes were used for analyses in this study. Parental line generation (G) G41* and G45* were used to examine number of ALVE integrations. Expression studies were performed in the brain and peripheral tissues of G45* birds. F1* birds of the reciprocal crosses were utilized to test inheritance of ALVE genes. Eighty-two F9* birds that form the advanced intercross were utilized for the correlation studies. QTL analyses have been performed with F2** and F8** birds in the advanced intercross line [16, 53]. B. Black bar represents a complete ALVE proviral genome. Grey bars indicate PCR primers and amplicons. C. Six SNPs between HWS and LWS lines were found in the 862 bp PCR fragment e* from both genomic DNA and cDNA. a*: pol197F/pol269R. b*: Val_envF/Val_envR. c*: env277F/env353R. d*: qPCR_envF/qPCR_envR. e*: an amplicon from a primer pair chENV232fwd/chENV1046rev. See table 1.Back to article page