Figure 3

MPMV transfer vectors RNA can be cross-packaged by MMTV proteins. (A) PCR amplification of DNase treated cytoplasmic RNAs using primers OTR537 and OTR538. (B) Control for nucleocytoplasmic RNA fractionation technique as described for figure 2B. (C) RT-PCR of cytoplasmic cDNA amplified using MPMV specific primers confirming that the transfer vector RNAs were stably expressed. (D) PCR amplification of DNase treated viral RNAs using viral specific primers. (E) RT-PCR of viral cDNAs amplified (for 20, 25, and 30 cycles) using virus specific primers and probed with the PCR product amplified using the same set of primers and pKAL011 as template. For this set of experiments, while amplifying DNase treated viral RNAs, cytoplasmic and viral cDNAs, primers OTR216 and OTR263 were used and should amplify 271 bp fragment.