Figure 5

Knockdown of CD81 enhances cell-to-cell transmission, but not cell-free infectivity. HeLa cells were infected with the indicated shRNA-carrying lentiviruses and transfected with pNL4-3. (A) Cell-free infectivity. Data shown represent the mean of 7 independent experiments (normalized to vector control) +/- SEM. (B) Cell-to-cell transmission. Data shown represent the mean of 4 independent experiments (normalized to vector control) +/- SEM. (C) Jurkat T cells were co-infected with shRNA-carrying lentiviruses and NL4-3. Data shown represent the mean of 4 independent experiments (normalized to vector control) +/- SEM. Cell-to-cell transmission was carried out as described in Methods. * denotes a statistically significant difference from scrambled shRNA (p < 0.05).