Figure 6

Nef augments bystander T cell death in HIV-infected HLAC. Summary of cell death analysis in HLAC infections as described in Fig. 5 from quadruplicate infections of HLAC from 5 donors. (A) Percentage of total productively infected CD4⁺ T lymphocytes. (B) Percentage of 7AAD-positive infected CD4⁺ T lymphocytes relative to wt that was arbitrarily set to 100%. (C, D) Percentage of dead uninfected CD4⁺ T lymphocytes relative to mock controls that were arbitrarily set to 100%. C depicts individual data points with the colour code identifying separate experiments, D the mean and SEM of these experiments. p-values indicate statistical significance based on Mann-Whitney-U analysis.