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Figure 9 | Retrovirology

Figure 9

From: Intracellular interactions between APOBEC3G, RNA, and HIV-1 Gag: APOBEC3G multimerization is dependent on its association with RNA

Figure 9

BiFC assays to evaluate interactions between HIV-1 Gag, A3G, and RNA-binding defective mutants of Gag and A3G. (E) BiFC assays to evaluate interactions between HIV-1 Gag and RNA-binding defective A3G mutants H65R, F70A, and Y91A. (F) BiFC assays to evaluate interactions between HIV-1 Gag NC mutants and A3G. (G) Quantification of YFP fluorescence reconstitution with HIV-1 Gag and A3G BiFC constructs using flow cytometry analysis. The percentage of YFP+ cells in mRFP+ cells after co-transfection with Gag-NY and Gag-CY (3.5%) was set to 100%. The error bars represent the s.e.m. of two independent experiments.

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Retrovirology

ISSN: 1742-4690

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