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Figure 7 | Retrovirology

Figure 7

From: TLR2 and TLR4 triggering exerts contrasting effects with regard to HIV-1 infection of human dendritic cells and subsequent virus transfer to CD4+T cells

Figure 7

Early steps in HIV-1 replication are modulated by TLR2 and 4 agonists. IM-MDDCs were either left untreated or stimulated for 2 hours with the TLR2 or 4 agonist. Cells were next pulsed with NL4-3/Balenv for 1 hour, washed twice and incubated at 37°C. Total DNA was extracted at 6, 24 or 48 hours post-infection and used for the detection and quantification of early reverse transcripts (A), late reverse transcripts (B) and integrated viral DNA (C) using a real-time PCR method. The number of HIV-1 copies was determined by a standard curve prepared with the NL4-3/Balenv vector. Data depicted in panels (A) and (B) represent the mean ± standard deviations of duplicate samples representative of two distinct donors whereas those illustrated in panel (C) represent the mean ± standard deviations of duplicate samples from 5 (Pam3Csk4) or 3 separate donors (LPS) (*: P < 0.05).

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Retrovirology

ISSN: 1742-4690

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