Intragenic transcriptional cis-activation of the human immunodeficiency virus 1 does not result in allele-specific inhibition of the endogenous gene

Table 1 Primers for RT-PCR and probes for RNA FISH used in this work.

Name	Sequence 5' > 3'
PRIMERS FOR RT-PCR
HIV_UP5	GGCGAGCCCTCAGATCCTGC
HIV_START	GGGTCTCTCTGGTTAGACCAGATCTGAGCC
HIV_RT	AGTCGCCGCCCCTCGCCTCCTGC
HIV_SPLICED	GGATTAACTGCGAATCGTTCTAGC
HIV_NUC	CGTCTGTTGTGTGACTCTGGTAACT
HMBOX_E1s	GTCTCTTTCCTCACTTCTTTCT
HMBOX_E1a	TCACAGTTTCCAGAACTCCAC
HMBOX_E2s	GGAATGGAACAGTGAAGAAGCA
HMBOX_E3s	AATGGTAGATAACGCAGATCATC
HMBOX_E3a	ACCACTGGAAAGGAACTAAGCA
PROBES FOR RNA FISH
HIV_MS2	AxGTCGACCTGCAGACAxGGGTGATCCTCAxGTTTTCTAGGCAATxA
HMBOX_E1a	AxAGTTTCCAGAAxTCCACACCGGAGACCCCACxTCCAGGATTCAAACCxT
HMBOX_E1b	CxCAGCGTCCGCxCACTTCCTCCCCAAAACCCCxCCAAAAAAATTGTTxT
HMBOX_I2a	AxGGTTGGTATAAACACAxAAAGCATGGTGGTxGTCTGGAGCTGGGGTTxA
HMBOX_I2b	AxTGCAGTGAGCCATGAxCACACCACAGTACxACAGCCTGGGTGATGAAxA
HMBOX_I2c	AxATTGCTGTCCTAAxCAGACTGCACCTGTGGxGTGGCTCTGACTGGTxA
HMBOX_I2d	AxGGTATGGTGGCAAAxCGACTCCCCCAGxACAACCACCAGAATATCAGxA
HMBOX_E4a	AxACGCCGAAGTCGCxGAAGCAGATCTATCTGCxCTATGGTAAATCTGGxA
HMBOX_E4b	AxATAACTGTTGCTAGGxGACGGGGACATTCCCGAAxGCTGCGTCTGTxA

Modified aminoallyl thymidines in the probes are indicated with "x". The sequence of one binding site for MS2 is underlined in the HIV_MS2 probe.

ISSN: 1742-4690