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Figure 2 | Retrovirology

Figure 2

From: Intragenic transcriptional cis-activation of the human immunodeficiency virus 1 does not result in allele-specific inhibition of the endogenous gene

Figure 2

A) HeLa cells were cotransfected with pHIV-Intro, pEYFP-MS2nls and either mDsRed tagged Tat (top) or Tat and mDsRed tagged Rev (bottom). Yellow spots in the nucleus correspond to nascent RNA from transfected plasmids. Cyan spots in the cytoplasm correspond to ECFPskl localized to peroxisomes. B) RT-PCR on HeLa cells transfected as indicated. Three primers were used, their position is shown in Figure 1. Resulting bands correspond to the unspliced and spliced HIV-Intro RNAs. Bottom panels: β-actin loading control (M = molecular weight marker). C) Effect of RNAPII inhibitors on HIV-Intro transcription in transfected HeLa cells transfected as indicated in Figure 2A, top panels. Nuclei showing transcription spots were scored 1 hour (gray bars) an 6 hours (black bars) after treatment with Actinomicin D (10 μg/ml), α-Amanitin (10 μg/ml) or Flavopiridol (500 μM).

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Retrovirology

ISSN: 1742-4690

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