Figure 3

(A) Inhibitory effects of SCTs expressing Tax180-188 on the growth of Tax-specific CTLs. An HTLV-I infected syngeneic rat cell line, FPM1.BP or various MOLT-4 cells were fixed with formalin and were then mixed with 4O1/C8. After 3 days of mixed culture, the growth of 4O1/C8 was evaluated using cell counting kit-8. (B) Production of IFN-γ in the culture supernatants was measured by ELISA after 3 days of mixed culture. (C) Apoptotic status of 4O1/C8 was evaluated by staining with Annexin V-FITC and anti-rat CD8 Ab-PE. (D) Production of IL-2 in the culture supernatants was measured by ELISA after 2 days of mixed culture. *P < 0.01, **P < 0.05, and ***P < 0.001 compared to the mixed culture with parental MOLT-4 cells. The data represent the mean ± the SD of triplicate wells. Similar results were obtained in two independent experiments.