The V1-V3 region of a brain-derived HIV-1 envelope glycoprotein determines macrophage tropism, low CD4 dependence, increased fusogenicity and altered sensitivity to entry inhibitors

Table 4 Analysis of Env-mediated fusogenicity^a.

	Log₁₀ [Y_max] (95% confidence limit)	Time to reach 1.0E+4 RLU/sec^b	Time to reach 1.0E+5 RLU/sec^b	Difference
Br	5.94 (5.76–6.12)	99	163	64
Spl	5.43 (5.01–5.85)	161	307	146
BS	5.83 (5.65–6.01)	106	178	72
SB	5.59 (5.49–5.70)	175	312	137
Br(N283T)	5.93 (5.74–6.12)	104	171	67
Spl(T283N)	5.50 (5.12–5.88)	175	323	148
Bv1v3	5.93 (5.71–6.14)	109	180	71
Bv1v2	5.62 (5.43–5.81)	137	243	106
DS17	5.65 (5.44–5.86)	125	220	95
DS17(N283T)	5.61 (5.32–5.89)	121	215	94
BaL	5.70 (5.51–5.89)	122	211	89

^aNon-linear regression analysis was performed using an exponential model (Y_max, estimated maximal response in RLU/second) with background-subtracted data comprising 60–420 minutes after initiation of co-culture.
^bEstimated time is expressed in minutes.

ISSN: 1742-4690