Figure 4

Treatment of HEK293 cells with proteasome inhibitor MG132 alleviates degradation of APOBEC3G and APOBEC3G-UBA2 fusion proteins. HEK293 cells were co-transfected as described in Fig. 2. Transfected cells were treated with 2.5 mM of the proteasome inhibitor MG132 27 hrs p.t. Western blot analysis was carried out as described in Fig. 3. B. The intensity of APOBEC3G protein and the value of the relative intensity of APOBEC3G were calculated the same way as described in Fig. 2.