| τ1 (ns) | α1 (%) | τ2 (ns) | α2 (%) | τ3 (ns) | α3 (%) | τ4 (ns) | α4 (%) | <τ> (ns) |
---|
Apo-Tat | 0.21 ± 0.03 | 25 ± 2 | 1.35 ± 0.01 | 35 ± 3 | 2.60 ± 0.20 | 19 ± 1 | 4.5 ± 0.2 | 21 ± 5 | 1.96 ± 0.08 |
Holo-Tat | 0.22 ± 0.05 | 18 ± 4 | 1.30 ± 0.20 | 37 ± 3 | 2.79 ± 0.09 | 25 ± 3 | 5.1 ± 0.2 | 20 ± 3 | 2.24 ± 0.07 |
- aExperiments were performed with 1.5 μM Tat proteins in 50 mM Hepes buffer, pH7.5, at 20°C. The lifetimes, τ
i
, and relative amplitudes, α
i
, are expressed as means for at least three independent experiments. The mean lifetimes were calculated with: ⟨τ⟩ = ∑α
i
τ
i
. The excitation and emission wavelengths for Trp were set at 295 nm and 350 nm, respectively.