Figure 4
HTLV-1 infection up-regulated expression of the PTHrP receptor (PTH1R) in PBMCs. PTHrP receptor expression and human β2M were measured by RT-PCR from total RNA at various time points in the co-culture assays. (A) Up-regulation of PTH1R in PBMCs at weeks 1, 3, 5, 7, 9, 11, and 13 following HTLV-1 infection in the presence or absence of IL-2 compared to day 0; controls 1 and 4 are PBMC-1 and PBMC-2; controls 2 and 5 are PBMC-1 and PBMC-2 stimulated with PHA; controls 3 and 6 are PBMC-1 and PBMC-2 stimulated with IL-2 for one week. ANOVA with Dunnett's tests were used to analyze the data from PTH1R RT-PCR quantification (bar graph shown at the bottom of the panel). The PTH1R levels were significantly greater at weeks 5, 7, 9, and 13 (p < 0.05; indicated by asterisks in the figure). (B) PTH1R expression in HTLV-1-infected T-cells and ATLL cells. Lanes represent: (1) Jurkat (2) MT-2 (3) SLB-1 (4) HUT102 (5) C8166 (6) MET-1 (7) RV-ATL (8) HT-1RV cells. The data showed that PTH1R expression was very low or absent in the ATLL cells (MET-1 and RV-ATL) compared to HTLV-1-infected T-cell lines (MT-2, SLB-1 and HT-1RV). Jurkat T-cells were used as a negative control. β2M was used a loading control.