Figure 5
From: Optimization of the doxycycline-dependent simian immunodeficiency virus through in vitro evolution
U3 and TAR mutations improve SIV-rtTA replication. (A) PM1 T-cells were transfected with 5 μg of the original (grey symbols) or LTR-mutated SIV-rtTA proviral plasmid (black symbols) and cultured with or without dox (closed and open symbols, respectively). Virus replication was monitored by measuring the reverse transcriptase level in the culture supernatant. (B) Cells were transfected with 1 μg SIV-rtTA or SIVmac239 proviral plasmid and cultured with dox (SIV-rtTA variants) or without dox (SIVmac239).