Figure 1

Design and expression of NC mutants used for the fine mapping of Stau1/NC interaction. (A) Schematic representation of Pr55^Gag with emphasis on the sequence of NC and its two zinc fingers. Several point mutations were introduced in the basic region or in the zinc fingers of NC-p1-YFP fusion protein to generate four mutants. (B) 293T cells were transfected with YFP, NC-p1-YFP and mutated NC-p1-YFP expressors. 48 hours post-transfection, cell lysates were prepared and analyzed by Western blotting using anti (α)-GFP antibodies.