Figure 5

ATP is neither affecting the T-cell stimulatory capacity of iDCs nor virus production in CD4⁺ T cells. (A) iDCs were either left untreated or treated for 60 min at 37°C with the indicated concentrations of ATP. Next, cells were extensively washed and co-cultured for 3 days with heterologous CD4⁺ T cells at a 1:10 ratio. Cellular proliferation was finally assessed using a colorimetric MTS assay. (B) iDCs were either left untreated or treated for 60 min at 37°C with the indicated concentrations of ATP. Next, iDCs were extensively washed and co-cultured with autologous CD4⁺ T cells that were previously pulsed with VSV-G-pseudotyped luciferase reporter viruses. Luciferase activity was assessed at 48 h following initiation of the co-culture. (C) CD4⁺ T cells were infected with NL4-3Balenv either in absence or presence of ATP (400 μM). Cells treated with the antiviral compound efavirenz (EFV) were used as controls. Virus production was monitored by measuring the cell-free p24 contents over time. Data shown represent the means ± standard deviations of triplicate samples and are representative of three independent experiments.