Figure 2

ATP affects virus transfer from iDCs but not mDCs. iDCs and mDCs from the same donor were first pulsed for 60 min at 37°C with R5-tropic JR-CSF either in absence or presence of ATP at the listed concentrations. Next, cells were washed extensively to remove input virus and co-cultured with autologous CD4⁺ T lymphocytes at a 1:3 ratio. Viral production was assessed at day 2 following initiation of the co-culture. Results from one representative experiment with triplicate samples are presented in panel A. The data shown in panel B represent the means ± standard deviations calculated from two independent experiments and are expressed as relative viral production compared to untreated cells. Asterisks denote statistically significant data (**, P < 0.01).