Figure 5

Toxicity of lime juice following topical application onto reconstructed cervicovaginal epithelia. Reconstructed cervicovaginal epithelial tissues (MatTek Corporation) were exposed to LiJ (diluted using normal saline) for 5, 30, 60 or 120 minutes. Juice was then removed by washing and tissue viability assessed by the method of MTT dye reduction. Data shown are expressed as percentage viability (when compared to an untreated control) andrepresent the mean ± SEM of n = 3 independent experiments. Statistical analysis was completed using ANOVA with Bonferroni post tests and statistically significant changes marked with * (p < 0.05) or ** (p < 0.01).