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Figure 2 | Retrovirology

Figure 2

From: Human Immunodeficiency Virus type-1 reverse transcriptase exists as post-translationally modified forms in virions and cells

Figure 2

RT isoforms are present in purified HIV virions. Viral particles from H3B cells were pelleted through 25% sucrose, solubilised in 2D gel electrophoresis buffer and an aliquot resolved by 1D SDS-PAGE (A). The remaining sample was spiked with 3 μg of GAPDH protein, focussed on a pH 7–11 non-linear, 11 cm Immobiline DryStrip gel and then resolved by SDS-PAGE followed by transfer to PVDF membranes (B). RT was detected by Western blot using an anti-RT antibody. RT isoforms (B) are designated by black arrows and the calculated pI and expected position of p66 and p51 indicated.

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Retrovirology

ISSN: 1742-4690

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