Figure 6
Lipid raft association of the SIV Env protein. The interaction of the Env protein of SIVmac239 (A), SIVmac239-EnvT (B), and SIVmac239-EnvC787S (C) with lipid rafts was analyzed in a discontinuous sucrose gradient. CEMx174 (A, B, C) cells were infected with 2 pfu/cell of respective vaccinia recombinant viruses. The infected cells were labeled with 35S-methionine/cysteine, disrupted by detergent TX-100 and a discontinuous sucrose gradient of 5 to 30% sucrose was used to obtain 11 fractions as described in Methods.