Figure 5
Production of SIV infectious particles in dividing and non-dividing CEMx174 cells. CEMx174 cells in a 96-well plate about 3 × 104 per well treated or untreated with aphidicolin were infected by SIV variants with the same titer determined as described in Methods. The supernatants were collected after 1 and 3 days post infection and the p27 content was determined by ELISA Core Antigen assay (Table 2). SIV particles with about 0.5 ng/well of p27 antigen were used for inoculation of JC-53B cells. The infectivity of particles was measured by removal of the media after 3 days, fixation and staining of cells with X-gal. The percent of particle infectivity was determined by dividing the number of infected cells in wells inoculated with particles collected from supernatants of SIV infected non-dividing cells by the number in wells inoculated with particles collected from supernatants of SIV infected dividing cells after 3 days (the maximum amount for each virus). Data are plotted as the mean of three experiments, each replicated twice.