Isolated HIV-1 core is active for reverse transcription
© Warrilow et al; licensee BioMed Central Ltd. 2007
Received: 28 August 2007
Accepted: 24 October 2007
Published: 24 October 2007
Whether purified HIV-1 virion cores are capable of reverse transcription or require uncoating to be activated is currently controversial. To address this question we purified cores from a virus culture and tested for the ability to generate authentic reverse transcription products. A dense fraction (approximately 1.28 g/ml) prepared without detergent, possibly derived from disrupted virions, was found to naturally occur as a minor sub-fraction in our preparations. Core-like particles were identified in this active fraction by electron microscopy. We are the first to report the detection of authentic strong-stop, first-strand transfer and full-length minus strand products in this core fraction without requirement for an uncoating activity.
Deoxyribonucleotides added directly to HIV-1 virions are incorporated into reverse transcription products [1–4]. This process, which is reported to disrupt the structure of the core in virions , is referred to as natural endogenous reverse transcription (NERT). Restructuring of the core also occurs post-infection when the core enters the cytoplasm after fusion of the viral envelope and is referred to as uncoating . One commonly accepted interpretation of NERT is that the observed virion disruption is analogous to uncoating, and uncoating may be a requirement for formation of an active reverse transcription complex (RTC) (reviewed in ).
An alternative corollary of the ability of intact virions to generate reverse transcription products is that cores purified from virions should be capable of reverse transcription. Whilst purified cores have been shown to contain reverse transcriptase [8–15], there is just one report of cores generating authentic reverse transcription products, but only when complemented with an "uncoating activity" from activated lymphocytes . The question of the biochemical state of virion core is of particular interest in the light of recent reports of reverse transcription in cores in vivo , and is important for our understanding of early replication events. To explore this controversial question, we used a modification of a commonly used method of core purification. We demonstrated that cores were able to generate authentic RT products without a requirement for an uncoating activity, as described below.
Core fractions have reverse transcription activity
Isolation of morphologically intact cores from HIV-1 particles has been reportedly improved by "spin-thru" methods [8, 18]. The principle of the method is that virions are delipidated by brief sedimentation through a detergent layer (0.03% Triton X-100). Free cores are separated from virions and debris by subjecting them to equilibrium gradient sedimentation on a continuous 20–60% Optiprep density gradient for 20 h; cores sediment to the dense layers (1.24 – 1.28 g/ml).
Western analysis and electron microscopy of core fractions
Western analysis was performed on gradient fractions to determine their composition. To provide sufficient material for analyses, a fresh equilibrium gradient scaled up approximately 20-fold was performed (Fig. 1C–F), and fractions were then analyzed by western analysis using purified anti-HIV-1 IgG (NIH AIDS Research and Reference Reagent Program). Multiple protein bands in the peak virus fractions 3 and 4 (1.08 and 1.15 g/ml, respectively) reacted with Gag proteins including capsid (Fig. 1C) as expected for intact virions. Only capsid protein was detected in the denser fractions 8 and 9 (density 1.26 and 1.30 g/ml, respectively), confirming our ELISA results. Reverse transcriptase was detected in these fractions by colorimetric ELISA using homopolymeric template (Fig. 1D); matrix was detected by western analysis using a specific monoclonal antibody (data not shown) as has been reported in other core preparations [11, 13, 14]; and gp41 was also detected in fractions 8 and 9 (Fig. 1E). A small amount of gp41 has been reported in cores purified using detergent . In that study, gp41 was attributed to microvesicles that co-purified with the cores. This seems unlikely as microvesicles are generally less dense than core . Alternatively, due to our novel virus culture method, our preparation may have contained a proportion of immature virions which are known to a have a stable association between gp41 and immature cores .
Transmission electron microscopy (TEM) was used to further characterize the denser fractions. Confirmation that the denser fractions of the untreated sample contained cores was obtained when numerous 80 – 100 nm cone and rod-shaped structures were observed in these fractions (Fig. 1F). No whole virions were observed. The above data are consistent with dense fractions with capsid and ERT activity which most likely contain biochemically active cores.
We are the first to report the detection of authentic strong-stop, first-strand transfer and full-length minus strand products in a core fraction. This confirms our expectations, from observations of the NERT reaction, that core is capable of reverse transcription, at least to full length minus-strand synthesis. It confirms that the enzymatic activities sufficient for reverse transcription are present in the core. Our data also support the suggestion that core may increase the effective concentration of components important for reverse transcription reaction, facilitating strand transfers and the efficiency of the overall reaction. The density of core does not sterically block polymerase elongation; however, we have no data as to the effect of elongation on core structure and it could be that the elongation of the polymerase results in shedding of capsid as suggested by the effect of NERT on virion morphology . Some cellular protein, perhaps the uncoating factor, may assist the elongating complex to efficiently complete reverse transcription.
Preparation of cores without detergent treatment to remove the viral envelope would appear to be counterintuitive. Interestingly, in support of our data, capsid protein has been reported in dense fractions of virions subjected to equilibrium gradient ultracentrifugation without prior detergent treatment , although the reverse transcription capacity was not assessed. One explanation for the presence of cores in our samples is that virions could have been gently disrupted by our culture and purification method, as core release by damage to virions has been reported . We chose to amplify virus on MAGI cells for 6 days prior to concentration on 20% sucrose cushion (see supplementary methods). This method may have been sufficiently disruptive to the envelope to result in core release.
Our data conflict with these previous observations of a requirement for an "uncoating activity" to activate reverse transcription activity . It is possible that cores prepared using detergent methods require complementation by a cell factor, perhaps an uncoating activity, to be activated. In contrast, we have found cores to be active for reverse transcription, at least making DNase I-resistant full length minus-strand DNA, albeit inefficiently, without requiring capsid release.
Our isolation of active cores without detergent treatment was fortuitous and reproducible; however, the quantity of the naturally-occurring core fraction varied from preparation to preparation. We, therefore, attempted to isolate cores by a more reliable method. Due to the denaturing effects of detergent, we attempted a number of other methods (data not shown) such as freeze-thaw treatment and exposure to β-cyclodextrin, which removes cholesterol and leads to lipid bilayer breakdown . To date none of these methods has resulted in reliable isolation of cores that are positive for ERT activity.
We have provided evidence for reverse transcription in a core fraction, and previous detergent experiments also suggest core structure is important for this process (Warrilow et al., manuscript under review). Whilst our data indicate a cell "uncoating activity" is not required to initiate reverse transcription or generate some late products, it is still consistent with a model in which the elongating RTC formation requires a cellular factor(s), for regulation of uncoating, or for completion of reverse transcription.
endogenous reverse transcription
CD4/CXCR4 expressing HeLa cells
natural endogenous reverse transcription
transmission electron microscopy.
- Hooker CW, Harrich D: The first strand transfer reaction of HIV-1 reverse transcription is more efficient in infected cells than in cell-free natural endogenous reverse transcription reactions. J Clin Virol. 2003, 26: 229-38. 10.1016/S1386-6532(02)00121-X.View ArticlePubMed
- Zhang H, Dornadula G, Alur P, Laughlin MA, Pomerantz RJ: Amphipathic domains in the C terminus of the transmembrane protein (gp41) permeabilize HIV-1 virions: a molecular mechanism underlying natural endogenous reverse transcription. Proc Natl Acad Sci U S A. 1996, 93: 12519-24. 10.1073/pnas.93.22.12519.PubMed CentralView ArticlePubMed
- Zhang H, Dornadula G, Pomerantz RJ: Endogenous reverse transcription of human immunodeficiency virus type 1 in physiological microenviroments: an important stage for viral infection of nondividing cells. J Virol. 1996, 70: 2809-24.PubMed CentralPubMed
- Zhang H, Dornadula G, Pomerantz RJ: Natural endogenous reverse transcription of HIV-1. J Reprod Immunol. 1998, 41: 255-60. 10.1016/S0165-0378(98)00062-X.View ArticlePubMed
- Zhang H, Dornadula G, Orenstein J, Pomerantz RJ: Morphologic changes in human immunodeficiency virus type 1 virions secondary to intravirion reverse transcription: evidence indicating that reverse transcription may not take place within the intact viral core. J Hum Virol. 2000, 3: 165-72.PubMed
- Nisole S, Saib A: Early steps of retrovirus replicative cycle. Retrovirology. 2004, 1: 9-10.1186/1742-4690-1-9.PubMed CentralView ArticlePubMed
- Goff SP: Intracellular trafficking of retroviral genomes during the early phase of infection: viral exploitation of cellular pathways. J Gene Med. 2001, 3: 517-28. 10.1002/1521-2254(200111)3:6<517::AID-JGM234>3.0.CO;2-E.View ArticlePubMed
- Accola MA, Ohagen A, Gottlinger HG: Isolation of human immunodeficiency virus type 1 cores: retention of Vpr in the absence of p6(gag). J Virol. 2000, 74: 6198-202. 10.1128/JVI.74.13.6198-6202.2000.PubMed CentralView ArticlePubMed
- Forshey BM, Aiken C: Disassembly of human immunodeficiency virus type 1 cores in vitro reveals association of Nef with the subviral ribonucleoprotein complex. J Virol. 2003, 77: 4409-14. 10.1128/JVI.77.7.4409-4414.2003.PubMed CentralView ArticlePubMed
- Forshey BM, von Schwedler U, Sundquist WI, Aiken C: Formation of a human immunodeficiency virus type 1 core of optimal stability is crucial for viral replication. J Virol. 2002, 76: 5667-77. 10.1128/JVI.76.11.5667-5677.2002.PubMed CentralView ArticlePubMed
- Kotov A, Zhou J, Flicker P, Aiken C: Association of Nef with the Human Immunodeficiency Virus Type 1 Core. J Virol. 1999, 73: 8824-8830.PubMed CentralPubMed
- Liu H, Wu X, Newman M, Shaw GM, Hahn BH, Kappes JC: The Vif protein of human and simian immunodeficiency viruses is packaged into virions and associates with viral core structures. J Virol. 1995, 69: 7630-8.PubMed CentralPubMed
- Ohagen A, Gabuzda D: Role of Vif in stability of the human immunodeficiency virus type 1 core. J Virol. 2000, 74: 11055-66. 10.1128/JVI.74.23.11055-11066.2000.PubMed CentralView ArticlePubMed
- Tang S, Murakami T, Cheng N, Steven AC, Freed EO, Levin JG: Human immunodeficiency virus type 1 N-terminal capsid mutants containing cores with abnormally high levels of capsid protein and virtually no reverse transcriptase. J Virol. 2003, 77: 12592-602. 10.1128/JVI.77.23.12592-12602.2003.PubMed CentralView ArticlePubMed
- Welker R, Hohenberg H, Tessmer U, Huckhagel C, Krausslich HG: Biochemical and structural analysis of isolated mature cores of human immunodeficiency virus type 1. J Virol. 2000, 74: 1168-77. 10.1128/JVI.74.3.1168-1177.2000.PubMed CentralView ArticlePubMed
- Auewarakul P, Wacharapornin P, Srichatrapimuk S, Chutipongtanate S, Puthavathana P: Uncoating of HIV-1 requires cellular activation. Virology. 2005, 337: 93-101. 10.1016/j.virol.2005.02.028.View ArticlePubMed
- Arhel NJ, Souquere-Besse S, Munier S, Souque P, Guadagnini S, Rutherford S, Prevost MC, Allen TD, Charneau P: HIV-1 DNA Flap formation promotes uncoating of the pre-integration complex at the nuclear pore. Embo J. 2007, 26: 3025-37. 10.1038/sj.emboj.7601740.PubMed CentralView ArticlePubMed
- Kotov A, Zhou J, Flicker P, Aiken C: Association of Nef with the human immunodeficiency virus type 1 core. J Virol. 1999, 73: 8824-30.PubMed CentralPubMed
- Bess JW, Gorelick RJ, Bosche WJ, Henderson LE, Arthur LO: Microvesicles are a source of contaminating cellular proteins found in purified HIV-1 preparations. Virology. 1997, 230: 134-44. 10.1006/viro.1997.8499.View ArticlePubMed
- Wyma DJ, Kotov A, Aiken C: Evidence for a stable interaction of gp41 with Pr55(Gag) in immature human immunodeficiency virus type 1 particles. J Virol. 2000, 74: 9381-7. 10.1128/JVI.74.20.9381-9387.2000.PubMed CentralView ArticlePubMed
- Kiernan RE, Ono A, Freed EO: Reversion of a human immunodeficiency virus type 1 matrix mutation affecting Gag membrane binding, endogenous reverse transcriptase activity, and virus infectivity. J Virol. 1999, 73: 4728-37.PubMed CentralPubMed
- Scholz I, Arvidson B, Huseby D, Barklis E: Virus particle core defects caused by mutations in the human immunodeficiency virus capsid N-terminal domain. J Virol. 2005, 79: 1470-9. 10.1128/JVI.79.3.1470-1479.2005.PubMed CentralView ArticlePubMed
- Graham DR, Chertova E, Hilburn JM, Arthur LO, Hildreth JE: Cholesterol depletion of human immunodeficiency virus type 1 and simian immunodeficiency virus with beta-cyclodextrin inactivates and permeabilizes the virions: evidence for virion-associated lipid rafts. J Virol. 2003, 77: 8237-48. 10.1128/JVI.77.15.8237-8248.2003.PubMed CentralView ArticlePubMed
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