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Figure 6 | Retrovirology

Figure 6

From: Requirements for the selective degradation of CD4 receptor molecules by the human immunodeficiency virus type 1 Vpu protein in the endoplasmic reticulum

Figure 6

Effect of a TDN mutant of p97 on Vpu-mediated CD4degradation. HEK 293T cells were mock-transfected or co-transfected with 1.5 μg of SVCMV CD4 wt, 12 μg of SVCMV Vpu- or Vpu+ and 1 μg of an expression plasmid encoding a FLAG-tagged version of p97 wt or the TDN mutant p97 AA. Cells were treated with BFA for 2 h prior to lysis. Cell lysates were then analyzed by western-blot as described in materials and methods. These results are representative of the data obtained in two independent experiments.

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Retrovirology

ISSN: 1742-4690

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