Figure 6

DC-SIGN mediated transfer of CQ passaged viruses and C33A derived viruses in the presence of CQ. Raji and Raji-DC-SIGN cells were incubated with viruses before washing with PBS and addition of CD4⁺ T-lymphocytes. CA-p24 levels were determined at day 7 by standard ELISA. The CA-p24 levels of transfer by Raji cells alone were subtracted from the CA-p24 values of transfer observed with Raji-DC-SIGN cells. A) DC-SIGN dependent transfer of viruses cultured long-term in the presence or absence of CQ (days 14, 77 and 197). B) DC-SIGN dependent transfer of JR-CSF, 299.10 and LAI virus produced in C33A cells either in the presence or absence of CQ. Standard deviations are depicted in both panels and P-values given.