Figure 5From: The role of the N-terminal segment of CCR5 in HIV-1 Env-mediated membrane fusion and the mechanism of virus adaptation to CCR5 lacking this segmentEnv(NYP) is more readily inactivated by treatment with sCD4 than Env(wt). Cells expressing either Env(wt) or Env(NYP) were collected from the culture dish using a non-enzymatic cell dissociation solution and treated with 25 μg/ml sCD4 for 30 min at 37°C. Cells were washed twice to remove free sCD4 and co-incubated with HeLa-CD4/CCR5(wt) cells for 2 hr at 37°C. The results are plotted as percentage of fusion observed for untreated effector cells.Back to article page