Figure 3
From: Intracellular HIV-1 Gag localization is impaired by mutations in the nucleocapsid zinc fingers
Plasma membrane accumulation of the HIV-1 NC(ΔZF1ZF2) Gag. HeLa cells were transfected with wild-type HIV-1 (A) or NC(ΔZF1ZF2) (B) DNA, then fixed and stained for the detection of Gag with an anti-MAp17; with an anti-CD63/Lamp3 for late endosomes, and with an anti-Lamp1 for lysosomes, as indicated. Zoomed-1 picture shows wild-type Gag colocalization with CD63/Lamp3 late endosomal marker (26 ± 6%) and zoomed-2 picture with the Lamp1 marker (6 ± 2%). In contrast, zoomed-3 picture shows less colocalization of this mutant with Lamp3 in comparison to wt (6 ± 4%). Zoomed-4 picture shows an accumulation of NC(ΔZF1ZF2)-Gag mutant at the PM, and less or equal with Lamp1(+) intracytoplasmic vesicles (3 ± 1%).