Figure 4From: Overlapping enhancer/promoter and transcriptional termination signals in the lentiviral long terminal repeatLentiviral siRNA silencing of NFκB reduces RNA readthrough of LV SIN LTR. The NF kB siRNA lentiviral vector was made by annealing two oligos pre-treated at 95°C for 5 min and gradually cooled down to room temperature. The annealed products were cloned into pTYF-Puro-hU6r LV-driven by a human U6 promoter. The NFκB silenced TE26 cell line was generated after puromycin selection and used for RNA TR analysis as described in the article. A. Real-time quantitative RT-PCR analysis of NFκB mRNA. B. Western analysis of NFκB expression in LV-NFκB siRNA-transduced TE26 cells. C. Comparison of RNA readthrough rate in TE26 and NFκB-silenced TE26 cells. The significance of difference is analyzed by paired student t test and shown by asterisks.Back to article page