Figure 3

In vitro analysis of viral transduction into a panel of immortalized cell lines. Advanced lentiviral vectors were used for transduction into various immortalized kidney-based cell lines. COS-7 (African green monkey), MDCK (dog), TCMK-1 and RAG (mouse) were transduced with VSV-G pseudotyped lentiviral vectors produced in 293T cells using three different transfer plasmids, pHRSVcPGKnlsLacZR(+)W(+) (Fig. 3A), pHRSVcPGKnlsLacZS1.4(+) (Fig. 3B) and pHRSVcPGKnlsLacZM4(+) (Fig. 3C). n = 5 different lentiviral preparations/cell line. * p < 0.05 difference between HeLa versus all of the other immortalized cell lines.