Figure 4

Diagram of antisense RNA derived from NY5 sequences originating from the HIVaINR, along with predicted amino acid sequence(s). The sequence varies from that of the HIV antisense gene inserted into HIV-AS-FLAG vector at the nucleotides indicated with a dot, and the alternative HAP amino acid is also indicated (discussed in text). Sites of postulated +1 ribosomal frameshifting are indicated with *. Stop codons are underlined and in the amino acid translation indicated with (X). The amino acids comprising the 4041 peptide are boxed, and the primers used for HIV antisense RNA detection are indicated (dashed arrows). Not shown is the run of four-five U bases embedded in a GC rich region encoded at the U3 terminus, which could potentially function as a termination signal (for either RNA polymerase 11 or 111) for this antisense RNA (compare B clade WEAU, or JRCSF with NY5) [18].