Figure 4
From: HIV-1 infection and CD4 T cell depletion in the humanized Rag2-/-γc-/-(RAG-hu) mouse model
PCR detection of HIV-1 in infected RAG-hu mice. Peripheral blood was collected from infected mice at different weeks post-infection. Cellular and plasma fractions were separated by centrifugation. DNA from the cellular fractions was subjected to DNA PCR to detect integrated virus (A), whereas the RNA extracted from the plasma fraction was subjected to RT-PCR to detect cell-free virus (B). Results from a representative HIV-1 infected RAG-hu mouse (#16) are shown.