Figure 6

An aliquot of RNA from the three donors for microarray analysis were reverse transcribed for quantitative real-time PCR assays for Cyclin T1, CDK9, and control α-tubulin mRNAs. Two sets of primers were designed to amplify different regions of Cyclin T1 mRNA (see Methods). Fold-change was calculated as the change in transcript levels in prostratin-treated cells relative to DMSO-treated cells after normalization to α-Tubulin levels.