| Mutation | LTR | NFkB |
---|
Mo | None | 100% | 100% |
 | G21D | 14% | 140% |
 | L87I | < 5% | 125% |
 | P92L | 13% | 120% |
 | Y144C | < 5% | < 5% |
 | Y144R | < 5% | < 5% |
 | T204A | 300% | 168% |
 | W248R | 10% | < 5% |
 | L308V | 75% | 80% |
2B | None | 246% | 195% |
Tax 1 | None | 100% | 100% |
 | Y144C | 55% | 36% |
 | W248R | < 5% | < 5% |
- Jurkat cells were co-transfected with the indicated Tax plasmids together with 1 ug of the LTR or NFkB luciferase reporters and 50 ng of the control Renilla plasmid pRL TK. After 24 hrs the cells were lysed and reporter activities were measured using a Turner 20/20 Luminometer and firefly reporter activities were normalised using Renilla luciferase values. Normalised values for wildtype Tax 2B and mutant Tax proteins were calculated as a percentage of the corresponding wildtype Mo or Tax 1 values which was set at 100%.