From: The retrovirus RNA trafficking granule: from birth to maturity
RTG Component | Cellular Function | Link to Retroviral Replication Cycle | Virion-Incorporation | Reference* |
---|---|---|---|---|
Actin | Cytoskeletal component for cell structure; scaffold for intracellular trafficking | Acts with HIV-1 Rev to promote nucleo-cytoplasmic RNA Transport; Binds retroviral Gags | Yes | [178–181] |
DDX1, 3, 5 | RNA Helicases involved in RNA splicing, nuclear RNA export, RNA translation) | Promotes genomic RNA Nucleocyto-plasmic export | ? | [113] |
EF1α | Translation elongation factor involved in RNA anchoring via the actin cytoskeleton and in RNA translation | Binds HIV-1 Gag and may inhibit Gag RNA translation to favour encapsidation | Yes | [182] |
eIF5A | Translation initiation factor involved in RNA translation initiation | A cofactor in Rev-mediated nucleocyto-plasmic RNA transport | ? | [178, 183] |
hnRNP A/B | Family of pre-mRNA splicing factors involved in RNA Splicing, RNA nucleocytoplasmic export, RNA trafficking | Influences retroviral RNA pre-mRNA splicing, RNA trafficking and gene expression | No | [34, 64, 133, 151] |
Hsp70 | Heat shock protein serving as a protein chaperone following heat or cellular stress | Promotes viral assembly, binds HIV-1 Vpr and promotes pre-integration complex nuclear nuclear import | Yes | [184–187] |
Kinesin-1** (KIF-5, KHC) | Molecular motor protein involved in energy-dependent intracellular translocation | The kinesin, KIF-4, interacts with retro-viral Gag Proteins | ? | [168, 169] |
Nucleolin | RNA binding or chaperone protein with numerous nuclear functions: remodeling of chromatin structure, ribosomal DNA transcription, ribosomal RNA maturation, ribosome assembly and nucleocytoplasmic transport | Binds genomic RNA of MLV and HIV-1 | Yes (MLV) | [188, 189] |
PABP1 | Poly(A) tail binding protein involved in RNA translation and RNA stability | Binds HIV-1 instability (INS) RNA sequences | ? | [190] |
PSF | Pre-mRNA splicing factor associated to polypyrimidine tract binding protein; Co-transcriptional RNA splicing | Interacts with HIV-1 cis-repressor (CRS) or INS RNA sequences | ? | [191] |
Pur1α | DNA- and RNA-binding protein involved in transcription and RNA transport | Binds TAR/Tat and can transactivate the HIV-1 LTR | ? | [192–194] |
RHA | RNA Helicase involved in RNA splicing and nuclear RNA export | Nucleocytoplasmic export and translation of genomic RNA | ? | [156, 157] & K. Boris-Lawrie, personal communication |
Staufen1*** | Double-stranded RNA-binding protein involved in RNA trafficking & metabolism | Binds pr55Gag and is in the genomic RNA ribonucleoprotein complex | Yes | [172, 175] |
tRNA synthetases | Enzyme catalyzing the synthesis of tRNAs for RNA translation | Binds retroviral Gag and is virion incorporated | Yes | [195, 196] |