Volume 2 Supplement 1

2005 International Meeting of The Institute of Human Virology

Open Access

HIV-1 Specific CD4 and CD8 T-cell Responses Associated With Low Viral Load in Treatment-Naïve HIV-1 Infected Individuals

  • Gunnel Biberfeld1Email author,
  • K Godoy-Ramirez1,
  • B Mäkitalo1,
  • R Thorstensson1,
  • C Nilsson1,
  • B Hejdeman1,
  • E Sandström1 and
  • H Gaines1
Retrovirology20052(Suppl 1):S62

DOI: 10.1186/1742-4690-2-S1-S62

Published: 8 December 2005

In preparation for monitoring of vaccine-induced responses, we determined HIV-specific cell-mediated immune responses in 17 treatment naïve HIV-1 infected individuals with > 400 CD4+ T cells/ml for at least 5 years including 9 patients with low viral load (VL, < 5000 copies/ml) and 8 with high VL (> 5000 copies/ml). HIV-1 specific IFN-γ-production and cytolytic activity were higher in subjects with low VL. The differences between the two groups were statistically significant for CD4+ T-cell responses to Gag and Nef peptides, tested by a long-term (48 h) ICS assay and of border-line significance for the Gag-specific cytolytic responses measured by a flow-cytometry assay and a chromium release assay. We also found a significant inverse correlation between VL and IFN-γ-production by CD8+ T-cells in response to Gag as measured by ICS. The ELISpot IFN-γ response was not significantly different in patients with high and low VL. During a median follow-up period of 2.4 years, 6 of 8 subjects with high VL and 1 of 9 with low VL showed decreasing CD4+ T-cell counts, and ARV treatment was more frequently initiated in the former patient group (5 of 8 versus 1 of 9). The CD4 and CD8 T cell immune responses found to be associated with low VL and stable CD4 counts may be of importance for protection.

Notes

Authors’ Affiliations

(1)
Swedish Institute for Infectious Disease control and Karolinska Institutet

Copyright

© The Author(s) 2005

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