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Figure 3 | Retrovirology

Figure 3

From: Targeted infection of HIV-1 Env expressing cells by HIV(CD4/CXCR4) vectors reveals a potential new rationale for HIV-1 mediated down-modulation of CD4

Figure 3

Stable transduction of Env+ cells by HIV-1-Neo(CD4/CXCR4) particles. (A) Vector particles isolated from Hek293 cells after single transfections of HIV-1-Neo or after co-transfections of HIV-1-Neo with DNAs encoding either CD4 or the five individual chimeric CD4 proteins or VSV G protein. (B) Particles were harvested after single transfection of HIV-1-Neo into HeLaT4 or HeLaS2 cells stably expressing CD4 or CD4/gpi proteins, respectively. Equal amounts of particles (1 ng p24) were used to infect either 105 Env+ TF228 or Env- BJAB cells and neomycin-resistant cell colonies were selected.

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Retrovirology

ISSN: 1742-4690

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