Figure 5

In vivo transduction of neural cells. Immunohistochemical labeling for PLAP (A) and the neuron-specific HU antigen (B) was used to identify pREV-HW3 transduced neurons in the cerebellum (5 dpi). Examples of cells expressing both antigens are indicated by white arrows. Cells expressing PLAP were identified on the peripheral extremity of the section (magenta arrowhead in A, B and C). Transduced cells were also found in the brain parenchyma (example indicated by white arrowhead). In all the regions examined transduced cells co-expressed both proteins as illustrated by immunodetection of both PLAP (D) and Neo (E) in cells in the IGL of the cerebellum (14 dpi). Co-expression of both antigens, PLAP (G) and Neo (H), also occurred in cells in the ventricular region such as in the ependymal walls of the 3rd ventricle (G, H and I). DNA is stained with bis-benzimide (C, F and I). PCL, purkinje cell layer; IGL, internal granular layer. Scale bars correspond to 150 μm (C and F), 75 μm (I)