Figure 3

Effect of p10-CA mutations on virus particle morphology. WT (top left and right), M239F (middle left and right), and M239G (bottom left and right) viruses from transfected cells were sedimented through 20% sucrose cushions, resuspended, and processed for electron microscopy. At low magnification (left; top, middle and bottom), WT and M239F cores appeared conical or bullet-shaped, whereas M239G cores sometimes appeared conical (left-bottom, leftmost virus), but more often appeared with large misshapen cores. At higher magnification (right; top, middle and bottom), internal cores were difficult to discern without significant adjustment of image contrast levels. Size bars for the two magnifications of images appear in bottom left and right panels, and correspond to 100 nm.