Figure 2

Time course of cell-surface PS levels and cell susceptibility to RD114 vector infection of NIH 3T3/ASCT2 and NIH 3T3 cells during treatment with PS. Cells were plated on day 0. 400 μM PS was added on day 1 at 24, 20, 16, 12, 8, and 4 h pre-infection. At the time of infection, cells were either infected with the RD114 vector [LAPSN(RD114)] or were assayed for cell-surface PS levels by using annexin-V. Top panel: annexin-V staining of NIH 3T3 cells was undertaken as described in Materials and Methods. Middle panel: LAPSN(RD114) infection of NIH 3T3/ASCT2 cells. Bottom Panel: LAPSN(RD114) infection of NIH 3T3 cells. Data points shown are means of duplicates, and each series represents an independent experiment. Data is represented as a percentage of the highest value observed.