Human immunodeficiency virus Tat associates with a specific set of cellular RNAs
© Bouwman et al.; licensee BioMed Central Ltd. 2014
Received: 4 December 2013
Accepted: 18 June 2014
Published: 3 July 2014
Human Immunodeficiency Virus 1 (HIV-1) exhibits a wide range of interactions with the host cell but whether viral proteins interact with cellular RNA is not clear. A candidate interacting factor is the trans-activator of transcription (Tat) protein. Tat is required for expression of virus genes but activates transcription through an unusual mechanism; binding to an RNA stem-loop, the transactivation response element (TAR), with the host elongation factor P-TEFb. HIV-1 Tat has also been shown to alter the expression of host genes during infection, contributing to viral pathogenesis but, whether Tat also interacts with cellular RNAs is unknown.
Using RNA immunoprecipitation coupled with microarray analysis, we have discovered that HIV-1 Tat is associated with a specific set of human mRNAs in T cells. mRNAs bound by Tat share a stem-loop structural element and encode proteins with common biological roles. In contrast, we do not find evidence that Tat associates with microRNAs or the RNA-induced silencing complex (RISC). The interaction of Tat with cellular RNA requires an intact RNA binding domain and Tat RNA binding is linked to an increase in RNA abundance in cell lines and during infection of primary CD4+ T cells by HIV.
We conclude that Tat interacts with a specific set of human mRNAs in T cells, many of which show changes in abundance in response to Tat and HIV infection. This work uncovers a previously unrecognised interaction between HIV and its host that may contribute to viral alteration of the host cellular environment.
KeywordsHuman immunodeficiency virus Tat RNA Transcription factor RNA immunoprecipitation Microarray T cell MicroRNA
Like all viruses, HIV-1 depends on the internal environment of the host cell to complete its life cycle and during virus replication a number of interactions occur between the virus and its host. These include interactions between viral and cellular proteins and viral proteins and host DNA. Whether HIV encodes proteins that allow interaction with cellular mRNA is unknown.
HIV Tat activates transcription of viral messenger RNA (mRNA) and genomic RNA from the integrated provirus and is necessary for HIV replication [1, 2]. In the absence of Tat, RNA polymerase II initiates transcription at the HIV long terminal repeat (LTR) but processive elongation is blocked, leading to the production of short RNA transcripts [3, 4]. This stalled transcriptional state also occurs in latently infected resting CD4+ T cells [4, 5] and is thought to underlie life-long virus persistence.
Tat localizes to the LTR through an unusual mechanism, binding to the trans-activation response element (TAR), an RNA stem loop formed in nascent viral transcripts [6–8]. Tat binds to TAR with the host elongation factor P-TEFb through direct interaction with the CyclinT1 subunit [9–12]. P-TEFb then phosphorylates the RNA polymerase II C-terminal domain and the repressive factors NELF and DSIF, allowing transcriptional elongation. Tat also directly binds and recruits histone acetyltransferases  and the nucleosome remodeling complex SWI/SNF [14, 15]. These changes in chromatin conformation allow full transcription of HIV messenger RNA (mRNA) and genomic RNA and subsequent release of infectious virions.
HIV Tat has also been suggested to function as a suppressor of post-transcriptional gene silencing (PTGS) [16–19], interacting with Dicer , but these findings have not been confirmed by other studies [20, 21]. Such activity may counter reported repression of HIV replication by the cellular PTGS pathway [16–19, 22–27]. HIV RNA has been observed to be recruited to cellular mRNA processing bodies (P-bodies)  and knockdown of RNA induced silencing complex (RISC) and P-body components [21–24], or inhibition of specific cellular miRNAs [25–27], have been described to increase HIV RNA abundance and viral replication. However, a more recent study did not observe co-localisation of HIV RNA with P-bodies or observe effects of P-body component knockdown on HIV replication .
In addition to binding to TAR, Tat has the potential to interact with host RNAs but whether this occurs in cells is unknown. Although the high affinity of Tat for TAR requires the stem-loop structure and a bulge of unpaired nucleotides present on the 5’ side of the loop, Tat can tolerate a range of mutations to the TAR sequence [29, 30] and this may allow interaction with host RNA to occur in cells. Consistent with this possibility, Tat has been reported to bind to 3 cellular mRNA molecules, all in vitro. Recombinant Tat binds to a predicted stem loop at the 5’ end of IL6 mRNA in vitro and this sequence is necessary for Tat-mediated increase in IL6 RNA in vivo. Similarly, recombinant Tat binds TLR4 RNA in vitro, Tat-containing cell lysate shifts a SOD2 RNA probe  and Tat-mediated activation of LTA (TNFβ) requires the presence of a predicted stem-loop structure at the 5’ end of its mRNA .
Expression of HIV Tat in cells leads to changes in cellular gene expression that often mirror the changes that occur during HIV infection [35–39]. Given that Tat has also been reported to interact with a wide range of cellular proteins, including components of the basal transcription machinery [40, 41], transcription factors [42, 43], chromatin regulators [44, 45], anti-viral factors [46, 47], and cell surface receptors [48, 49], it is unclear whether effects of Tat on the levels of cellular mRNAs reflect direct Tat-RNA interactions.
Considering that Tat is an RNA binding protein and that it can alter cellular RNA abundance, we hypothesized that Tat binding to human RNA might represent an uncharacterized interaction between HIV and its host. We report here experiments revealing that HIV Tat associates with a specific set of cellular mRNAs in T cells.
Tat associates with human mRNA in T cells
We next sought to verify the array results by measuring enrichment of individual RNAs by Q-PCR (Figure 2C). We noted that RNAs encoding ISG20 and the apoptosis mediators TNFRSF8 (CD30) and FADD, previously shown to be upregulated by HIV Tat [36, 39], were bound by Tat in both array experiments. QPCR for TNFRSF8, FADD and ISG20 mRNA in replicate HA and FLAG Tat IPs confirmed enrichment of these RNAs compared to GAPDH mRNA. Also consistent with the array data, qPCR for HPRT1 mRNA showed that this was not enriched compared to GAPDH mRNA (between 0.2-1-fold). The arrays therefore accurately report enrichment of specific mRNAs in Tat IP fractions.There is a potential with native RNA IP experiments that proteins form new interactions with RNA after cell lysis that did not occur in the intact living cell. To test whether Tat also interacted with human RNAs within intact cells, we repeated the RNA IP after crosslinking the cells with formaldehyde (Figure 2D). These experiments showed that FADD and TNFRSF8 remain enriched in IPs from crosslinked cells and therefore that Tat interacts with these RNAs in living cells.
Tat-associated human RNAs are enriched for specific functions
We next used Gene Ontology to explore whether the set of RNAs that interact with Tat had any functions in common (Figure 2E). To provide robust statistics for this assessment, we used cluster analysis to identify a larger set of 2000 RNAs enriched in both HA and FLAG RNA IPs and a control set of 2000 RNAs depleted in HA and FLAG IPs. Gene categories specifically enriched in the set of Tat-associated RNAs included nucleotide binding and the overlapping categories transferase activity, tRNA metabolic process and ligase activity. Indeed, this set of RNAs included 26 of the 34 enzymes that constitute the KEGG aminoacyl-tRNA biosynthesis pathway (Additional file 2). The enrichment of mRNAs encoding shared functional annotations suggests that they may share specific sequence or structural features or a common RNA binding protein that mediates interaction with HIV Tat.
Tat does not associate with miRNAs
The interaction of Tat with cellular RNA depends on its RNA binding domain
Enrichment of a stem-loop structure within Tat-bound mRNAs
Human RNAs bound by Tat increase in abundance during HIV infection of primary CD4+ T cells
We sought to characterise the two clusters of upregulated RNAs further to understand the significance of the association of Tat primarily with the second, later cluster. Gene Ontology revealed that genes in the first cluster upregulated by 14 hours have functions in the response to virus (5-fold enriched, p = 0.002), with the categories immune response (2.2-fold enriched, p = 0.0014) and regulation of programmed cell death (2.9-fold, p = 2.7×10-5) also enriched (Figure 6B and Additional file 5). Comparison with expression data from T cells exposed to interferon β showed that this cluster corresponds to the classical cellular interferon response to virus infection (Figure 6C).
In contrast, genes in the second cluster more strongly associated with Tat are instead enriched for RNAs encoding proteins with roles in ribosome biogenesis (19-fold enrichment, p = 6×10-9), RNA metabolic process (8-fold enrichment, p = 1.2×10-4) and mitotic cell cycle (6-fold enrichment, p = 1.4×10-5) (Figure 6B and Additional file 5). Contrary to their upregulation by HIV, these genes are instead downregulated by interferon (Figure 6C) and thus upregulation would appear to be specific for HIV infection. We considered that upregulation of these genes normally repressed by interferon might reflect an ability of Tat to counteract the normal interferon-stimulated response. However, gene expression changes induced by interferon in Tat-expressing CEM cells were similar to those in the parental cell line (Additional file 6). We conclude that Tat interacts with a specific set of cellular RNAs that are upregulated during HIV infection.
Interaction with HIV Tat is associated with an increase in RNA abundance
The upregulation of Tat-associated genes during HIV infection suggests that this increase in RNA abundance occurs due to Tat. To test this, we examined the abundance of mRNAs in CEM cells stably expressing Tat compared with the parental CEM cell line and a CEM line stably expressing GFP, selected in parallel. We found that although the majority of Tat-associated mRNAs did not show changes in abundance in cells expressing Tat, significantly more RNAs exhibited an increase in abundance than expected by chance (n = 23, p < 0.001, Hypergeometric; Figure 6D, Additional file 7). RNAs bound by Tat that also increased upon expression of Tat included TNFRSF8, ISG20, MAPK12, METRNL and DNAJB2, all of which are also upregulated during HIV infection (Additional file 4, [35–39]). In contrast, there was no significant relationship between Tat binding and decreases in transcript abundance (n = 5, p = 0.95, Figure 6D). Furthermore, there was no relationship between the RNAs detected in control IPs and changes in RNA abundance in Tat-expressing cells (p = 0.178).We next considered whether Tat RNA binding activity was necessary for increases in RNA abundance. We constructed two stable cell lines, in which expression of either WT Tat or the Tat RNA binding mutant K50S-K51G could be induced by treatment with Doxycycline and measured RNA abundance using gene expression microarrays (Figure 6E). We found that cells expressing WT Tat exhibited changes in RNA abundance compared with cells expressing Tat K50S-K51G, with a greater number of genes showing increased expression in the WT Tat cells (Figure 6E). Furthermore, changes in RNA abundance were correlated with detection of Tat binding to the RNA, with Tat being bound more frequently to the set of RNAs exhibiting increased abundance in WT Tat cells compared to RNAs that exhibited decreased abundance. We conclude that Tat RNA binding is required for Tat to increase RNA abundance.
Although many interactions between HIV and host cell proteins have been described, whether the virus interacts with cellular RNAs was unknown. We have discovered that HIV Tat interacts with a set of cellular mRNAs in human T cells. These interactions are specific because they can be detected using two different epitope tags, they are not observed in control IPs, and the interactions can be verified by Q-PCR. The interaction of Tat with cellular RNA depends on lysine residues within the RNA binding domain known to be important for interaction with TAR. We could not detect association of Tat with miRNAs or with protein components of RISC. The cellular mRNAs bound by Tat are associated with specific molecular functions and share predicted stem-loop elements. Tat expression and HIV infection frequently results in an increase in the abundance of Tat-bound RNAs.
Tat has previously been shown to interact with three human RNAs by gel-shift assays in vitro[31–33] but such experiments lack specificity and whether these few interactions are representative of those occurring in cells was not clear. The system we have developed, in which RNA is purified from immunoprecipitated Tat tagged with independent epitopes and expressed from its own promoter, allowed us to detect the specific interactions between physiological levels of Tat and cellular RNA as they occur in cells. Indeed, although these experiments show that Tat interacts with a select set of human RNAs in cells, we could not detect an interaction between Tat and the RNAs with which it has previously been reported to interact in vitro[31–33].
Tat binding to TAR tolerates mutations to the stem and loop and, although the bulge structure at position +23 is required, Tat recognises a number of variants [29, 30]. Tat also binds related structures predicted to form within the IL6 and TLR4 mRNAs [31, 32]. Analysis of motifs significantly enriched in the set of Tat-bound RNAs revealed a striking palindromic GC rich motif that is predicted to fold into stem-loop structures similar to TAR (Figure 5). We found that these motifs were located throughout Tat target RNAs, rather than being located in at the 5’ end as for TAR. Therefore, if Tat does recognise such RNA elements, they are unlikely to function in the same way as TAR in the activation of transcriptional elongation. Identification of Tat-RNA UV-crosslink sites at high-resolution using sequencing-based methods such as iClip or PAR-Clip  will confirm whether Tat is binding to these elements and whether this occurs in live cells. Their importance for Tat binding could then be tested through the expression of RNAs in which such structures are disrupted by mutation.
Tat has previously been shown to cause changes to human gene expression and some of these changes have been linked to HIV pathology [35–39]. How Tat mediates such changes in RNA abundance is unclear. In addition to being an RNA binding protein, Tat has also been reported to interact with a great many proteins, from transcription factors to cell surface receptors [40–49], and so Tat-mediated changes in gene expression could theoretically be due to these interactions rather than Tat RNA binding function. Arguing against this, we have identified a global association between Tat binding to cellular mRNAs and an increase in RNA abundance. Firstly, a number of RNAs previously shown to be upregulated by HIV and by Tat are also bound by Tat, including ISG20, JUN, DNAJB2, ISG20, MAPK12, METRNL, TNFRSF8 and OAS3. Secondly, Tat is associated with a cluster of RNAs upregulated during HIV infection. Thirdly, we detect Tat binding at a significant number of the RNAs that increase in abundance in Tat-expressing cells. Finally, mutations that block Tat RNA binding activity counteract these increases in RNA abundance. Thus, the association of Tat with specific mRNAs provides a direct link between Tat and changes in RNA abundance during HIV infection that is parsimonious with its core RNA binding function. Tat binding to host RNA may act to recruit or displace a cellular factor or otherwise alter mRNA transcription, processing, export or stability. Further experiments will be required to identify the mechanism through which Tat RNA binding leads to changes in cellular mRNA abundance.
Early studies of Tat function considered the hypothesis that Tat might counteract the host interferon response  and there is some evidence Tat can regulate interferon target genes. Treatment of cells with recombinant Tat has been suggested to suppress IFNγ signaling through upregulation of SOCS2  and Tat has been reported to interact with PKR, modulating its function . Expression of Tat in dendritic cells leads to induction of interferon-inducible genes encoding T cell chemo-attractants, luring these target cells to sites of infection . We thus examined whether Tat bound to interferon-regulated RNAs, and whether the presence of Tat altered the transcriptional response to interferon-β in our T cell line model. However, we did not find evidence that Tat RNA binding acts to modulate the transcriptional response to interferon (Additional file 6).
Although we could detect interaction of Tat with TAR and cellular mRNAs and detect interaction of Ago2 with miRNAs, we could not detect interaction of Tat with cellular miRNAs. This suggests that Tat either does not interact with miRNAs or that the interaction occurs at a low level beyond our detection. Consistent with the lack of detected Tat-miRNA interactions, we also failed to identify an interaction between Tat and endogenous Dicer, Ago1 or Ago2. Tat has previously been reported to directly interact with exogenously expressed Myc-tagged Dicer , but interaction with the endogenous protein has not been described. Whether or not Tat inhibits RNA silencing is also unclear; some studies report that Tat can repress small RNA generation [16–19], but others have been unable to confirm this [20, 21]. Our data indicate that if Tat does act to inhibit PTGS, it is unlikely to do so through an association with cellular miRNAs.
Our discovery that Tat targets a specific set of cellular RNAs presents a new interface through which HIV can interact with its host. In addition to Tat, HIV encodes two other RNA binding proteins, Rev and nucleocapsid, which may also interact with cellular RNAs in addition to their viral targets. A number of other viruses encode RNA binding proteins that may also target specific cellular RNAs. For example, Hepatitis C virus (HCV) NS5a binds to the 3’-ends of HCV plus and minus strand RNAs and functions in genomic RNA replication but has also been implicated in the regulation of host genes, including upregulation of IL8 and LTB[65, 66]. Influenza NS1 interacts with viral mRNAs encoding M1 and NS1 but can also interact with polyA RNA, dsRNA and human U6 and U6atac snRNAs, functioning to inhibit pre-mRNA splicing, 3’-end processing and mRNA export (reviewed in ). Epstein-Barr virus (EBV) SM protein binds to unspliced viral RNA to allow export from the nucleus but also interacts with cellular STAT1 mRNA, inducing a splicing reaction that leads to production of the STATβ isoform that can act as a dominant-negative suppressor of STAT1α . The methods we have presented here could be used to determine whether these viral factors and others also interact with a specific set of host RNAs.
The mRNAs bound by Tat encode a set of proteins enriched for specific functions including nucleotide binding and tRNA metabolic processes. It is unclear why HIV Tat should target these RNAs in particular but similar functional classes of gene are also upregulated upon HIV infection (Figure 6B and Additional file 5). This may reflect the virus’s need to create an environment in which large amounts of viral genomic RNA and proteins can be efficiently synthesised. As such, the interaction of Tat with specific cellular RNAs may have been selected during evolution because it aids HIV replication. Alternatively, interactions between Tat and cellular RNAs may instead represent off-target effects of Tat’s TAR binding function that offers no selective advantage to the virus. Either way, the interaction of Tat with cellular RNA likely has consequences for the host cell. Further studies will be required to determine whether the association of Tat with specific cellular RNAs promotes virus replication or pathogenesis.
We have discovered that HIV Tat interacts with a specific set of cellular mRNAs. These interactions are detected regardless of the antibody used to purify Tat and occur when Tat is expressed at physiological levels. Tat does not interact with cellular miRNAs or with RISC components. Tat binding is linked to increases in mRNA abundance, both in Tat-expressing cells and during HIV infection of primary CD4+ T cells. The interaction of Tat with human RNA reveals a new interaction between HIV and its host. Given Tat’s pleiotropic effects on the host cell, this has implications for the pathology of this important viral agent. The interaction between viral proteins and host RNA may in future prove to be a common feature of virus infection.
Cells lines and plasmids
The promoter in pIRESneo3 was replaced by the LTR from HIV SF2 and HA or FLAG-tagged 101-amino acid Tat (from SF2) inserted downstream (before IRES-G418R). These constructs were transfected into CEM cells and stable lines selected. For RNA IP experiments measuring the role of Tat RNA binding function, HA-Tat was cloned into the pHR-SIN-CSGW-EGFP lentiviral expression construct  in which the EGFP cassette was replaced by IRES-emGFP and K50S-K51G mutations made by site-directed mutagenesis (Stratagene). The vectors were transfected into 293T cells together with the packaging plasmids pMDG and p8.91 and virus harvested, concentrated and titred on CEM cells. CEM cells were infected with the viruses with an MOI of 10. To quantify the expression in CEM cells relative to HIV infected cells, the HA tag was also inserted into the 5’ end of Tat in the p9B18 clone of HIV strain SF2. To compare changes in RNA abundance associated with Tat RNA binding activity, HA-Tat and HA-Tat K50S-K51G were cloned into pTRE2Hyg (Clontech), transfected into Jurkat Tet-On cells and stable lines selected.
HIV infection of primary CD4+ T cells
pNL4-3 encoding the NL4-3 strain of HIV was transfected into 293T cells and virus purified by pelleting through sucrose and titred on GHOST cells. CD4+ T cells were purified from PBMC (Buffy coat) by negative selection (Miltenyi), activated with anti-CD3 and anti-CD28 (1ug/ml) for 60 hours and then expanded in IL2 (5 ng/ml) for 48 hours. Cells were then split and infected with unpurified NL4-3 (contained within 293T cell supernatant), NL4-3 purified on a sucrose cushion (both with a dose of 10 infectious units per cell) or mock-infected by addition of 293-conditioned media. Virus was spinoculated at 1000 g for 1 hour and infection was allowed to proceed for 1 hour at 37°C. Virus was then washed off the cells and the cells incubated in fresh media. Cells were harvested in TRIzol (Invitrogen) at 14, 24 and 48 hrs after infection. We used purified HIV virus in addition to non-purified virus so we could filter out expression changes in the recipient T-cells potentially induced by cytokines secreted by the producer 293T cells. In fact, we didn’t observe any significant differences in the response to the two viral stocks and so both datasets were used in the analysis. After activation, non-infected primary CD4+ T cells were also treated by addition of interferon-beta (400 U/ml) for 4 hours.
CEM cells expressing HA or FLAG-tagged Tat were lysed (10 mM Hepes, 100 mM KCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, Complete protease inhibitor (Roche) and RNaseOUT (Invitrogen)) and the nuclei sheared through a 27-guage needle and by freeze-thawing, as described . The lysates were then DNase treated (Ambion) on ice for 30 mins, diluted in NT buffer (50 mM Tris pH7.5, 150 mM NaCl, 1 mM MgCl2, 0.05% NP-40, supplemented as before) and insoluble material removed by centrifugation. Lysates were precleared with protein-G beads (Dynal) and Tat IP performed with anti-HA (3F10; Roche) or anti-FLAG (M2; Sigma) antibody. Beads were washed 4 times with NT2 and twice with NT2 containing 1 M urea. Total RNA was purified from beads and input cell lysate with Trizol LS (Invitrogen), DNaseI treated and the integrity verified using an Agilent Bioanalyzer.
CEM cells crosslinked with 1% formaldehyde were lysed in ChIP lysis buffer  in the presence of RNaseOUT, sonicated at 9 W for 3 mins (20s pulses) and insoluble material removed by centrifugation. The lysate was precleared and then incubated with 3F10 or control Rat IgG (Santa Cruz) for 16 hours. The beads were washed as described  and the crosslinks reversed by incubation at 65°C for 1 hour. RNA was then purified with TRIzol LS and DNaseI treated.
Microarray analysis of RNA IP samples
200 ng of IP or input RNA were labelled using Agilent’s QuickAmp labelling kit and hybridized to DNA microarrays (Agilent). Initial HA-Tat and FLAG-Tat IPs were hybridized to a custom microarray containing multiple probes to all RefSeq mRNAs (includes standard Agilent probes) . For these custom arrays, RefSeq mRNAs with at least one probe with an IP/input ratio above 2-fold and p-value < 0.001 and at least one other with an IP/input ratio above 1.5-fold and p-value < 0.001 were judged to be enriched by the IP. Subsequent dye-swap and control IPs were analysed using catalogue Agilent 44K human gene expression arrays (AMADID 014850). For these arrays, the ratio and p-values were averaged for RefSeq mRNAs with multiple probes and then those mRNAs with an IP/input ratio above 2 and p-value < 0.001 were judged to be enriched by the IP. Log2 IP/input ratios were filtered to remove those with insignificant p-values and the remaining data clustered using average-linkage hierarchical clustering. A total of 3 Tat RNA IP microarray experiments were performed.
100 μg of input and IP RNA was labelled and hybridized to Agilent human miRNA arrays following the standard protocol. The log2 ratio of the miRNA signal to the geometric mean signal across all samples was calculated and samples and miRNAs clustered by average-linkage hierarchical clustering.
Gene expression analysis
Total RNA was purified from cells using Trizol, DNase treated (Ambion) and integrity confirmed. The HIV samples were hybridized together with reference RNA (Stratagene). The expression ratio between infected cells and untreated cells at the same timepoint was calculated, data for genes with multiple probes averaged and probes filtered for those exhibiting at least 1 absolute log2 expression ratio above 1. The CEM cell line material was hybridized together with RNA from the parental CEM line. Genes were judged to be differentially expressed if HA-Tat/CEM and HA-Tat/GFP were both above 1.5 or both below 0.66. Tat expression was induced in Jurkat cells with 1 μg/ml Doxycycline for 48 hours and hybridised with RNA from untreated cells. Gene Ontology analysis was performed using DAVID .
cDNA was synthesized with Superscript II (Invitrogen) and random primers. Primer and probes were from Applied Biosystems; FADD (Hs00356603_g1), ISG20 (Hs00158122_m1), TNFRSF8 (Hs01114493_m1) and HPRT1 (4333768) or custom synthesized; TAR (F:GCTAACTAGGGAACCCACTGCTT, R: CAACAGACGGGCACACACTACT, Probe: AGCCTCAATAAAGCTTGCCTTGAGTGCTTC), GAPDH (F: GGCTGAGAACGGGAAGCTT, R:AGGGATCTCGCTCCTGGAA, Probe: TCATCAATGGAAATCCCATCACCA), 7SK RNA (F:AGAACGTAGGGTAGTCAAGC, R:AGAAAGGCAGACTGCCACAT).
Co-immunoprecipitation, cell fractionation and immunoblotting
Cells were washed in PBS and lysed with 40 mM Tris pH 7.5, 150 mM NaCl, 10% glycerol, 0.3% NP-40 containing Complete protease inhibitors (Roche) for 20 min, and insoluble material removed by centrifugation. Supernatants were incubated with anti-HA (3F10) antibody bound to magnetic beads at 4°C for 16 hrs. Beads were then washed successively in IP buffer, IP buffer containing 300 mM NaCl, IP buffer containing 500 mM NaCl 0.1% NP-40, and then with 20 mM Tris–HCl pH 8.0, 150 mM NaCl, 10% glycerol. Lysates were separated by SDS-PAGE and proteins detected with the following antibodies: HA-Tat (Roche 3F10), FLAG (Sigma M2), TBP (Abcam ab818), Tubulin (Sigma T4026), Dicer (Abcam ab14601), Ago1 (Millipore 07–599) and Ago2 (Millipore 07–590). CEM cells were fractionated in nuclear and cytoplasmic fractions as described .
The set of 317 enriched mRNAs in both HA and Tat RNA IP were filtered to contain one transcript per gene (the longest transcript was selected) and run through MEME-ChIP  with the default options, except for scan given strand only, any number of repetitions per sequence, maximum width of 60, and compare to the motif database of Ray et al. . The distribution of motifs across the RNAs was determined using MAST , with an E-value of 1 and the options: use individual sequence composition, ENSEMBL homo sapiens database, scale motif display threshold and no treatment of reverse complement strands. To identify sequences within the bound RNAs that match the discovered motifs, we used FIMO  with the ENSEMBL homo sapiens database and scan given strand only option. We then selected the 143 sequences matching with motif 0 with a p-value of <10-9, or the 58 sequences that matched motif 2 with p < 10-6, and aligned these with LocARNA, using the global option . The consensus structures identified by LocARNA were then visualised with Pseudoviewer .
Availability of supporting data
The data sets supporting the results of this article are available in the ArrayExpress repository, accession number E-MEXP-3199.
Thanks to Dan Frampton for parsing the custom array data and to Greg Towers for critical reading of the manuscript. This work was funded by an MRC Career Development Award (G0802068) to RGJ and Wellcome Trust support to PK and was supported by the National Institute for Health Research University College London Hospitals Biomedical Research Centre. The authors also acknowledge support from the MRC Centre for Medical Molecular Virology.
- Dayton AI, Sodroski JG, Rosen CA, Goh WC, Haseltine WA: The trans-activator gene of the human T cell lymphotropic virus type III is required for replication. Cell. 1986, 44: 941-947.View ArticlePubMedGoogle Scholar
- Fisher AG, Feinberg MB, Josephs SF, Harper ME, Marselle LM, Reyes G, Gonda MA, Aldovini A, Debouk C, Gallo RC, Wong-Staal F: The trans-activator gene of HTLV-III is essential for virus replication. Nature. 1986, 320: 367-371.View ArticlePubMedGoogle Scholar
- Kao SY, Calman AF, Luciw PA, Peterlin BM: Anti-termination of transcription within the long terminal repeat of HIV-1 by tat gene product. Nature. 1987, 330: 489-493.View ArticlePubMedGoogle Scholar
- Adams M, Sharmeen L, Kimpton J, Romeo JM, Garcia JV, Peterlin BM, Groudine M, Emerman M: Cellular latency in human immunodeficiency virus-infected individuals with high CD4 levels can be detected by the presence of promoter-proximal transcripts. Proc Natl Acad Sci U S A. 1994, 91: 3862-3866.PubMed CentralView ArticlePubMedGoogle Scholar
- Lassen KG, Bailey JR, Siliciano RF: Analysis of human immunodeficiency virus type 1 transcriptional elongation in resting CD4+ T cells in vivo. J Virol. 2004, 78: 9105-9114.PubMed CentralView ArticlePubMedGoogle Scholar
- Muesing MA, Smith DH, Capon DJ: Regulation of mRNA accumulation by a human immunodeficiency virus trans-activator protein. Cell. 1987, 48: 691-701.View ArticlePubMedGoogle Scholar
- Feng S, Holland EC: HIV-1 tat trans-activation requires the loop sequence within tar. Nature. 1988, 334: 165-167.View ArticlePubMedGoogle Scholar
- Berkhout B, Silverman RH, Jeang KT: Tat trans-activates the human immunodeficiency virus through a nascent RNA target. Cell. 1989, 59: 273-282.View ArticlePubMedGoogle Scholar
- Mancebo HS, Lee G, Flygare J, Tomassini J, Luu P, Zhu Y, Peng J, Blau C, Hazuda D, Price D, Flores O: P-TEFb kinase is required for HIV Tat transcriptional activation in vivo and in vitro. Genes Dev. 1997, 11: 2633-2644.PubMed CentralView ArticlePubMedGoogle Scholar
- Zhu Y, Pe’ery T, Peng J, Ramanathan Y, Marshall N, Marshall T, Amendt B, Mathews MB, Price DH: Transcription elongation factor P-TEFb is required for HIV-1 tat transactivation in vitro. Genes Dev. 1997, 11: 2622-2632.PubMed CentralView ArticlePubMedGoogle Scholar
- Wei P, Garber ME, Fang SM, Fischer WH, Jones KA: A novel CDK9-associated C-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA. Cell. 1998, 92: 451-462.View ArticlePubMedGoogle Scholar
- Bieniasz PD, Grdina TA, Bogerd HP, Cullen BR: Recruitment of cyclin T1/P-TEFb to an HIV type 1 long terminal repeat promoter proximal RNA target is both necessary and sufficient for full activation of transcription. Proc Natl Acad Sci U S A. 1999, 96: 7791-7796.PubMed CentralView ArticlePubMedGoogle Scholar
- Marzio G, Tyagi M, Gutierrez MI, Giacca M: HIV-1 tat transactivator recruits p300 and CREB-binding protein histone acetyltransferases to the viral promoter. Proc Natl Acad Sci U S A. 1998, 95: 13519-13524.PubMed CentralView ArticlePubMedGoogle Scholar
- Mahmoudi T, Parra M, Vries RG, Kauder SE, Verrijzer CP, Ott M, Verdin E: The SWI/SNF chromatin-remodeling complex is a cofactor for Tat transactivation of the HIV promoter. J Biol Chem. 2006, 281: 19960-19968.View ArticlePubMedGoogle Scholar
- Treand C, du Chene I, Bres V, Kiernan R, Benarous R, Benkirane M, Emiliani S: Requirement for SWI/SNF chromatin-remodeling complex in Tat-mediated activation of the HIV-1 promoter. EMBO J. 2006, 25: 1690-1699.PubMed CentralView ArticlePubMedGoogle Scholar
- Bennasser Y, Le SY, Benkirane M, Jeang KT: Evidence that HIV-1 encodes an siRNA and a suppressor of RNA silencing. Immunity. 2005, 22: 607-619.View ArticlePubMedGoogle Scholar
- Bennasser Y, Jeang KT: HIV-1 Tat interaction with Dicer: requirement for RNA. Retrovirology. 2006, 3: 95-PubMed CentralView ArticlePubMedGoogle Scholar
- Haasnoot J, de Vries W, Geutjes EJ, Prins M, de Haan P, Berkhout B: The Ebola virus VP35 protein is a suppressor of RNA silencing. PLoS Pathog. 2007, 3: e86-PubMed CentralView ArticlePubMedGoogle Scholar
- Qian S, Zhong X, Yu L, Ding B, de Haan P, Boris-Lawrie K: HIV-1 Tat RNA silencing suppressor activity is conserved across kingdoms and counteracts translational repression of HIV-1. Proc Natl Acad Sci U S A. 2009, 106: 605-610.PubMed CentralView ArticlePubMedGoogle Scholar
- Lin J, Cullen BR: Analysis of the interaction of primate retroviruses with the human RNA interference machinery. J Virol. 2007, 81: 12218-12226.PubMed CentralView ArticlePubMedGoogle Scholar
- Sanghvi VR, Steel LF: A Re-Examination of Global Suppression of RNA Interference by HIV-1. PLoS One. 2011, 6: e17246-PubMed CentralView ArticlePubMedGoogle Scholar
- Nathans R, Chu CY, Serquina AK, Lu CC, Cao H, Rana TM: Cellular microRNA and P bodies modulate host-HIV-1 interactions. Mol Cell. 2009, 34: 696-709.PubMed CentralView ArticlePubMedGoogle Scholar
- Huang J, Wang F, Argyris E, Chen K, Liang Z, Tian H, Huang W, Squires K, Verlinghieri G, Zhang H: Cellular microRNAs contribute to HIV-1 latency in resting primary CD4+ T lymphocytes. Nat Med. 2007, 13: 1241-1247.View ArticlePubMedGoogle Scholar
- Triboulet R, Mari B, Lin YL, Chable-Bessia C, Bennasser Y, Lebrigand K, Cardinaud B, Maurin T, Barbry P, Baillat V, Reynes J, Corbeau P, Jeang KT, Benkirane M: Suppression of microRNA-silencing pathway by HIV-1 during virus replication. Science. 2007, 315: 1579-1582.View ArticlePubMedGoogle Scholar
- Chable-Bessia C, Meziane O, Latreille D, Triboulet R, Zamborlini A, Wagschal A, Jacquet JM, Reynes J, Levy Y, Saib A, Bennasser Y, Benkirane M: Suppression of HIV-1 replication by microRNA effectors. Retrovirology. 2009, 6: 26-PubMed CentralView ArticlePubMedGoogle Scholar
- Ahluwalia JK, Khan SZ, Soni K, Rawat P, Gupta A, Hariharan M, Scaria V, Lalwani M, Pillai B, Mitra D, Brahmachari SK: Human cellular microRNA hsa-miR-29a interferes with viral nef protein expression and HIV-1 replication. Retrovirology. 2008, 5: 117-PubMed CentralView ArticlePubMedGoogle Scholar
- Houzet L, Klase Z, Yeung ML, Wu A, Le SY, Quinones M, Jeang KT: The extent of sequence complementarity correlates with the potency of cellular miRNA-mediated restriction of HIV-1. Nucleic Acids Res. 2012, 40: 11684-11696.PubMed CentralView ArticlePubMedGoogle Scholar
- Phalora PK, Sherer NM, Wolinsky SM, Swanson CM, Malim MH: HIV-1 replication and APOBEC3 antiviral activity are not regulated by P bodies. J Virol. 2012, 86: 11712-11724.PubMed CentralView ArticlePubMedGoogle Scholar
- Cordingley MG, LaFemina RL, Callahan PL, Condra JH, Sardana VV, Graham DJ, Nguyen TM, LeGrow K, Gotlib L, Schlabach AJ, Colonno RJ: Sequence-specific interaction of Tat protein and Tat peptides with the transactivation-responsive sequence element of human immunodeficiency virus type 1 in vitro. Proc Natl Acad Sci U S A. 1990, 87: 8985-8989.PubMed CentralView ArticlePubMedGoogle Scholar
- Roy S, Delling U, Chen CH, Rosen CA, Sonenberg N: A bulge structure in HIV-1 TAR RNA is required for Tat binding and Tat-mediated trans-activation. Genes Dev. 1990, 4: 1365-1373.View ArticlePubMedGoogle Scholar
- Ambrosino C, Ruocco MR, Chen X, Mallardo M, Baudi F, Trematerra S, Quinto I, Venuta S, Scala G: HIV-1 Tat induces the expression of the interleukin-6 (IL6) gene by binding to the IL6 leader RNA and by interacting with CAAT enhancer-binding protein beta (NF-IL6) transcription factors. J Biol Chem. 1997, 272: 14883-14892.View ArticlePubMedGoogle Scholar
- O’Hara SP, Small AJ, Gajdos GB, Badley AD, Chen XM, Larusso NF: HIV-1 Tat protein suppresses cholangiocyte toll-like receptor 4 expression and defense against Cryptosporidium parvum. J Infect Dis. 2009, 199: 1195-1204.PubMed CentralView ArticlePubMedGoogle Scholar
- Flores SC, Marecki JC, Harper KP, Bose SK, Nelson SK, McCord JM: Tat protein of human immunodeficiency virus type 1 represses expression of manganese superoxide dismutase in HeLa cells. Proc Natl Acad Sci U S A. 1993, 90: 7632-7636.PubMed CentralView ArticlePubMedGoogle Scholar
- Buonaguro L, Buonaguro FM, Giraldo G, Ensoli B: The human immunodeficiency virus type 1 Tat protein transactivates tumor necrosis factor beta gene expression through a TAR-like structure. J Virol. 1994, 68: 2677-2682.PubMed CentralPubMedGoogle Scholar
- Sharma V, Knobloch TJ, Benjamin D: Differential expression of cytokine genes in HIV-1 tat transfected T and B cell lines. Biochem Biophys Res Commun. 1995, 208: 704-713.View ArticlePubMedGoogle Scholar
- Izmailova E, Bertley FM, Huang Q, Makori N, Miller CJ, Young RA, Aldovini A: HIV-1 Tat reprograms immature dendritic cells to express chemoattractants for activated T cells and macrophages. Nat Med. 2003, 9: 191-197.View ArticlePubMedGoogle Scholar
- Kim N, Dabrowska A, Jenner RG, Aldovini A: Human and simian immunodeficiency virus-mediated upregulation of the apoptotic factor TRAIL occurs in antigen-presenting cells from AIDS-susceptible but not from AIDS-resistant species. J Virol. 2007, 81: 7584-7597.PubMed CentralView ArticlePubMedGoogle Scholar
- Dabrowska A, Kim N, Aldovini A: Tat-induced FOXO3a is a key mediator of apoptosis in HIV-1-infected human CD4+ T lymphocytes. J Immunol. 2008, 181: 8460-8477.PubMed CentralView ArticlePubMedGoogle Scholar
- Lopez-Huertas MR, Callejas S, Abia D, Mateos E, Dopazo A, Alcami J, Coiras M: Modifications in host cell cytoskeleton structure and function mediated by intracellular HIV-1 Tat protein are greatly dependent on the second coding exon. Nucleic Acids Res. 2010, 38: 3287-3307.PubMed CentralView ArticlePubMedGoogle Scholar
- Kashanchi F, Piras G, Radonovich MF, Duvall JF, Fattaey A, Chiang CM, Roeder RG, Brady JN: Direct interaction of human TFIID with the HIV-1 transactivator tat. Nature. 1994, 367: 295-299.View ArticlePubMedGoogle Scholar
- Veschambre P, Roisin A, Jalinot P: Biochemical and functional interaction of the human immunodeficiency virus type 1 Tat transactivator with the general transcription factor TFIIB. J Gen Virol. 1997, 78: 2235-2245.View ArticlePubMedGoogle Scholar
- Jeang KT, Chun R, Lin NH, Gatignol A, Glabe CG, Fan H: In vitro and in vivo binding of human immunodeficiency virus type 1 Tat protein and Sp1 transcription factor. J Virol. 1993, 67: 6224-6233.PubMed CentralPubMedGoogle Scholar
- Yang Y, Dong B, Mittelstadt PR, Xiao H, Ashwell JD: HIV Tat binds Egr proteins and enhances Egr-dependent transactivation of the Fas ligand promoter. J Biol Chem. 2002, 277: 19482-19487.View ArticlePubMedGoogle Scholar
- Weissman JD, Brown JA, Howcroft TK, Hwang J, Chawla A, Roche PA, Schiltz L, Nakatani Y, Singer DS: HIV-1 tat binds TAFII250 and represses TAFII250-dependent transcription of major histocompatibility class I genes. Proc Natl Acad Sci U S A. 1998, 95: 11601-11606.PubMed CentralView ArticlePubMedGoogle Scholar
- Rohr O, Lecestre D, Chasserot-Golaz S, Marban C, Avram D, Aunis D, Leid M, Schaeffer E: Recruitment of Tat to heterochromatin protein HP1 via interaction with CTIP2 inhibits human immunodeficiency virus type 1 replication in microglial cells. J Virol. 2003, 77: 5415-5427.PubMed CentralView ArticlePubMedGoogle Scholar
- McMillan NA, Chun RF, Siderovski DP, Galabru J, Toone WM, Samuel CE, Mak TW, Hovanessian AG, Jeang KT, Williams BR: HIV-1 Tat directly interacts with the interferon-induced, double-stranded RNA-dependent kinase, PKR. Virology. 1995, 213: 413-424.View ArticlePubMedGoogle Scholar
- Brand SR, Kobayashi R, Mathews MB: The Tat protein of human immunodeficiency virus type 1 is a substrate and inhibitor of the interferon-induced, virally activated protein kinase. PKR J Biol Chem. 1997, 272: 8388-8395.View ArticlePubMedGoogle Scholar
- Brake DA, Debouck C, Biesecker G: Identification of an Arg-Gly-Asp (RGD) cell adhesion site in human immunodeficiency virus type 1 transactivation protein, tat. J Cell Biol. 1990, 111: 1275-1281.View ArticlePubMedGoogle Scholar
- Albini A, Soldi R, Giunciuglio D, Giraudo E, Benelli R, Primo L, Noonan D, Salio M, Camussi G, Rockl E, Bussolino F: The angiogenesis induced by HIV-1 tat protein is mediated by the Flk-1/KDR receptor on vascular endothelial cells. Nat Med. 1996, 2: 1371-1375.View ArticlePubMedGoogle Scholar
- Sobhian B, Laguette N, Yatim A, Nakamura M, Levy Y, Kiernan R, Benkirane M: HIV-1 Tat assembles a multifunctional transcription elongation complex and stably associates with the 7SK snRNP. Mol Cell. 2010, 38: 439-451.PubMed CentralView ArticlePubMedGoogle Scholar
- Klase Z, Kale P, Winograd R, Gupta MV, Heydarian M, Berro R, McCaffrey T, Kashanchi F: HIV-1 TAR element is processed by Dicer to yield a viral micro-RNA involved in chromatin remodeling of the viral LTR. BMC Mol Biol. 2007, 8: 63-PubMed CentralView ArticlePubMedGoogle Scholar
- Ouellet DL, Plante I, Landry P, Barat C, Janelle ME, Flamand L, Tremblay MJ, Provost P: Identification of functional microRNAs released through asymmetrical processing of HIV-1 TAR element. Nucleic Acids Res. 2008, 36: 2353-2365.PubMed CentralView ArticlePubMedGoogle Scholar
- Klase Z, Winograd R, Davis J, Carpio L, Hildreth R, Heydarian M, Fu S, McCaffrey T, Meiri E, Ayash-Rashkovsky M, Gilad S, Bentwich Z, Kashanchi F: HIV-1 TAR miRNA protects against apoptosis by altering cellular gene expression. Retrovirology. 2009, 6: 18-PubMed CentralView ArticlePubMedGoogle Scholar
- Ouellet DL, Vigneault-Edwards J, Letourneau K, Gobeil LA, Plante I, Burnett JC, Rossi JJ, Provost P: Regulation of host gene expression by HIV-1 TAR microRNAs. Retrovirology. 2013, 10: 86-PubMed CentralView ArticlePubMedGoogle Scholar
- Ruben S, Perkins A, Purcell R, Joung K, Sia R, Burghoff R, Haseltine WA, Rosen CA: Structural and functional characterization of human immunodeficiency virus tat protein. J Virol. 1989, 63: 1-8.PubMed CentralPubMedGoogle Scholar
- Machanick P, Bailey TL: MEME-ChIP: motif analysis of large DNA datasets. Bioinformatics. 2011, 27: 1696-1697.PubMed CentralView ArticlePubMedGoogle Scholar
- Ray D, Kazan H, Cook KB, Weirauch MT, Najafabadi HS, Li X, Gueroussov S, Albu M, Zheng H, Yang A, Na H, Irimia M, Matzat LH, Dale RK, Smith SA, Yarosh CA, Kelly SM, Nabet B, Mecenas D, Li W, Laishram RS, Qiao M, Lipshitz HD, Piano F, Corbett AH, Carstens RP, Frey BJ, Anderson RA, Lynch KW, Penalva LOF, et al: A compendium of RNA-binding motifs for decoding gene regulation. Nature. 2013, 499: 172-177.PubMed CentralView ArticlePubMedGoogle Scholar
- Bailey TL, Gribskov M: Combining evidence using p-values: application to sequence homology searches. Bioinformatics. 1998, 14: 48-54.View ArticlePubMedGoogle Scholar
- Grant CE, Bailey TL, Noble WS, FIMO: Scanning for occurrences of a given motif. Bioinformatics. 2011, 27: 1017-1018.PubMed CentralView ArticlePubMedGoogle Scholar
- Will S, Joshi T, Hofacker IL, Stadler PF, Backofen R, LocARNA-P: Accurate boundary prediction and improved detection of structural RNAs. RNA. 2012, 18: 900-914.PubMed CentralView ArticlePubMedGoogle Scholar
- König J, Zarnack K, Luscombe NM, Ule J: Protein-RNA interactions: new genomic technologies and perspectives. Nat Rev Genet. 2012, 13: 77-83.View ArticlePubMedGoogle Scholar
- Popik W, Pitha PM: Transcriptional activation of the tat-defective human immunodeficiency virus type-1 provirus: effect of interferon. Virology. 1992, 2: 435-447.View ArticleGoogle Scholar
- Cheng SM, Li JC, Lin SS, Lee DC, Liu L, Chen Z, Lau AS: HIV-1 transactivator protein induction of suppressor of cytokine signaling-2 contributes to dysregulation of IFNγ signaling. Blood. 2009, 113: 5192-5201.View ArticlePubMedGoogle Scholar
- Clerzius G, Gélinas JF, Gatignol A: Multiple levels of PKR inhibition during HIV-1 replication. Rev Med Virol. 2011, 21: 42-53.View ArticlePubMedGoogle Scholar
- Girard S, Shalhoub P, Lescure P, Sabile A, Misek DE, Hanash S, Bréchot C, Beretta L: An altered cellular response to interferon and up-regulation of interleukin-8 induced by the hepatitis C viral protein NS5A uncovered by microarray analysis. Virology. 2002, 295: 272-283.View ArticlePubMedGoogle Scholar
- Maqbool MA, Imache MR, Higgs MR, Carmouse S, Pawlotsky JM, Lerat H: Regulation of hepatitis C virus replication by nuclear translocation of nonstructural 5A protein and transcriptional activation of host genes. J Virol. 2013, 87: 5523-5539.PubMed CentralView ArticlePubMedGoogle Scholar
- Hale BG, Randall RE, Ortín J, Jackson D: The multifunctional NS1 protein of influenza A viruses. J Gen Virol. 2008, 89: 2359-2376.View ArticlePubMedGoogle Scholar
- Verma D, Swaminathan S: Epstein-Barr Virus SM Protein Functions as an Alternative Splicing Factor. J Virol. 2008, 82: 1780-7188.View ArticleGoogle Scholar
- Demaison C, Parsley K, Brouns G, Scherr M, Battmer K, Kinnon C, Grez M, Thrasher AJ: High-level transduction and gene expression in hematopoietic repopulating cells using a human immunodeficiency virus type 1-based lentiviral vector containing an internal spleen focus forming virus promoter. Hum Gene Ther. 2002, 13: 803-813.View ArticlePubMedGoogle Scholar
- Kanhere A, Viiri K, Araujo CC, Rasaiyaah J, Bouwman RD, Whyte WA, Pereira CF, Brookes E, Walker K, Bell GW, Pombo A, Fisher AG, Young RA, Jenner RG: Short RNAs are transcribed from repressed polycomb target genes and interact with polycomb repressive complex-2. Mol Cell. 2010, 38: 675-688.PubMed CentralView ArticlePubMedGoogle Scholar
- Dennis G, Sherman BT, Hosack DA, Yang J, Gao W, Lane HC, Lempicki RA: DAVID: Database for Annotation, Visualization, and Integrated Discovery. Genome Biol. 2003, 4: P3-View ArticlePubMedGoogle Scholar
- Zaitseva L, Cherepanov P, Leyens L, Wilson SJ, Rasaiyaah J, Fassati A: HIV-1 exploits importin 7 to maximize nuclear import of its DNA genome. Retrovirology. 2009, 6: 11-PubMed CentralView ArticlePubMedGoogle Scholar
- Byun Y, Han K: PseudoViewer3: generating planar drawings of large-scale RNA structures with pseudoknots. Bioinformatics. 2009, 25: 1435-1437.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.