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Table 1 The b12 susceptibility of 32 AE-Env clones before and after removing N186 and/or N197

From: Impact of amino acid substitutions in the V2 and C2 regions of human immunodeficiency virus type 1 CRF01_AE envelope glycoprotein gp120 on viral neutralization susceptibility to broadly neutralizing antibodies specific for the CD4 binding site

    

IC50of b12 (μg/ml)b

 
 

PNLG sitea

 

Mutation(s)c

 

Env clonea

N186

N197

Wild-type

N186Q

N197Q

N186Q/N197Q

29CC1

+ a

+

>40d

>40

0.10

8.34

45PB1

+

+

>40

>40

>40

1.60

45CC1

+

+

>40

>40

0.02

0.65

47PL1

- a

+

>40

 

0.03

 

55PL1

-

+

>40

 

21.90

 

62PL1

+

+

>40

>40

>40

0.77

65CC4

+

+

>40

1.31

  

99PB2

-

+

>40

 

3.65

 

99CC8

-

+

>40

 

0.10

 

101PL1

+

+

>40

>40

>40

12.98

102CC2

-

+

>40

 

12.20

 

105PB1

+

+

>40

>40

0.07

0.16

105PL2

-

+

>40

 

0.29

 

105PL3

+

+

>40

>40

0.03

0.24

107CC2

+

+

>40

0.02

  

21PL2

-

+

>40

 

>40

 

22PL1

-

+

>40

 

>40

 

41PB3

+

+

>40

>40

>40

>40

41CC1

+

+

>40

>40

>40

>40

47CC11

-

+

>40

 

>40

 

50PB2

-

+

>40

 

>40

 

50PL1

-

+

>40

 

>40

 

52PB3

-

+

>40

 

>40

 

52PL4

-

+

>40

 

>40

 

52PL7

+

+

>40

>40

>40

>40

60PB2

-

+

>40

 

>40

 

60PL2

-

+

>40

 

>40

 

60CC3

+

+

>40

>40

>40

>40

98CC2

-

+

>40

 

>40

 

98CC3

-

+

>40

 

>40

 

99PL2

-

+

>40

 

>40

 

104PB4

-

+

>40

 

>40

 
  1. aPNLG sites at the amino acid positions 186 (N186) and 197 (N197) were present (+) or absent (-) in the wild type of AE-Env clones. Amino acid numbering is based on the HXB2 Env gp120.
  2. bIC50 of b12 for suppressing viral replication was calculated using GraphPad Prism 5 software. Data are shown as the means of at least two independent experiments.
  3. cSingle or multiple amino acid mutations were introduced into AE-Env-recombinant viruses.
  4. dIC50 is >40 μg/ml.
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Retrovirology

ISSN: 1742-4690

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