Figure 6

Cytokines or LPS are not able to recapitulate the effect of Vpx in single round infection of MDM. MDM were infected with equal amounts of p24 of YU-2 and subtype B full-length viruses CH077 and CH058 for 6 h. Cells were washed and new medium was added along with interferon beta (IFN-β; 5000U/ml), interferon gamma (IFN-γ; 20 ng/ml), LPS (100 ng/ml), tumor necrosis factor (TNF-α ; 10 ng/ml), MIP-1α (0.1 μg/ml), or CCL19 (100 ng/ml). This is a representative example of at least two independent experiments.