Table 1 Thermodynamic parameters of isothermal titration calorimetry measurements

a.) SH3 _B6 –Nef–sdAb19 complex formation

SH3_B6 to Nef_SF2^b

18.7

−10.55

−22.51 (±0.25)

−0.040

−11.96

0.81 (±0.005)

sdAb19 to Nef_SF2

38.6

−10.11

−11.62 (±0.005)

−0.005

−1.51

0.81 (±0.002)

b.) sdAb19 binding to Nef

sdAb19 to myrNef_SF2

32.7

−10.20

−14.97 (±0.22)

−0.016

−4.77

0.81 (±0.006)

sdAb19 to Nef_NL4–3

18.5

−10.37

−17.97 (±0.30)

−0.025

−7.42

0.88 (±0.007)

sdAb19 to Nef_NA7

118

−9.45

−12.82 (±0.12)

−0.011

−3.34

0.74 (±0.004)

sdAb19 to Nef_SF2 (∆ flex. loop)

98

−9.55

−10.71 (±0.06)

−0.004

−1.16

0.87 (±0.003)

c.) sdAb19 mutant binding

sdAb19 (G102R,S103E) to Nef_SF2

920

−8.25

−9.93 (±0.56)

−0.006

−1.68

0.9 (±0.085)

sdAb19 (D60R) to Nef_SF2

3700

−7.40

−3.38 (±0.56)

0.014

4.02

0.74 (±0.009)

sdAb19 (triple) to Nef_SF2

–

–

–

–

–

–

d.) Nef _SF2 mutant binding

sdAb19 to Nef_SF2 (K148E)

1700

−7.86

−8.77 (±0.26)

−0.003

−0.89

0.74 (±0.015)

sdAb19 to Nef_SF2 (M198K)

–

–

–

–

–

–

sdAb19 to Nef_SF2 (L202K)

–

–

–

–

–

–

e.) Neffin binding to Nef

Neffin to Nef_SF2

1.6

−12.02

−38.26 (±0.17)

−0.088

−26.23

0.66 (±0.001)

Neffin to Nef_NL4–3

3.9

−11.49

−33.19 (±0.20)

−0.073

−21.71

0.65 (±0.002)

Neffin to Nef_NA7

14.4

−10.72

−34.69 (±0.12)

−0.080

−23.97

0.67 (±0.001)

Neffin (triple) to Nef_SF2

23.3

−10.41

−18.13 (±0.12)

−0.026

−7.72

1.08 (±0.004)