Figure 7

Expression of TAR and TAR miRNAs may not protect Jurkat cells from apoptosis. A) Apoptosis of Jurkat NEG-1 and Jurkat-TAR cell clones 1, 2 and 3 was induced by treating with etoposide (100 μM) or CH11 anti-fas activating antibody (25 ng/ml) for 6 hours. Cells were analyzed using a luminescent assay for Caspase 3 and 7 activity. Data are presented as fold increases of relative luminescent units compared to cells treated with 1% DMSO. B and C) Apoptosis of J-Lat cell clones 6.3, 8.4 and 10.6 was induced by treatment with etoposide (100 μM) or CH11 anti-fas activating antibody (25 ng/ml) for 6 hours. B) Cells were lysed and proteins were separated on a 10% polyacrylamide gel and analyzed by Western blot using antibodies against PARP-1 and α-tubulin. C) For Caspase 3 and 7 activities, the assay was performed according to the manufacturer’s instructions and luminescence detection was assessed. The results of three (3) independent experiments are shown separately.