| % activity of mutants relative to wild type (average of three experiments)a | ||||||
---|---|---|---|---|---|---|---|
Mutation | Binding to INI1 | Infection in CEM-GFP (day 10) | Infection in HeLa (24Â hr.) | Early RT at 6Â hr | Late RT at 6Â hr | 2LTR circle formation (at 12Â hr.) | Integration (at 24Â hr.) |
IN (WT) | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
H12Y | 20.60 | 0.12 | 0.07 | 11.12 | 7.15 | 0 | 0 |
Q137R | 10.00 | 0.09 | 0.40 | 29.89 | 12.10 | 30.62 | 5.42 |
D202G | 32.00 | 0.09 | 0.14 | 14.71 | 14.74 | 48.58 | 1.34 |
K71R | 46.00 | 3.74 | 2.52 | 38.13 | 27.73 | 150.78 | 61.64 |
S147G | 77.00 | 29.83 | 6.93 | 74.01 | 124.82 | 164.75 | 33.29 |
K111E | ND | 29.26 | 4.68 | 71.25 | 104.25 | 47.07 | 65.30 |
D116A | ND | 0.11 | 3.36 | 79.39 | 60.15 | 13369.26 | 1.44 |