Figure 5From: The S40 residue in HIV-1 Gag p6 impacts local and distal budding determinants, revealing additional late domain activitiesDisrupting Alix interaction with S40F restores spherical particle formation. COS-1 cells were transfected with plasmids expressing HA-tagged -P7L (panels A and D), P7L-S40F (panels B and E), or P7L-Y36S-S40F (panels C and F). Panels C-F, cells were prepared for examination by immunoelectron microscopy as described in Methods. Bars in panels A, and B measure 500Â nm; bars in panels C, D and F measure 100Â nm; bar in panel E measures 2000Â nm.Back to article page