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Figure 6 | Retrovirology

Figure 6

From: A murine leukemia virus with Cre-LoxP excisible coding sequences allowing superinfection, transgene delivery, and generation of host genomic deletions

Figure 6

Southern analysis of subclones. Cellular DNA digested with BsrG1 and visualized using an (a) EYFP and (b) Cre probe. Lanes contained DNA (8 μg/lane) from clones (1) AYGC.2A2, (2) AYGC.2A3, (3) AYGC.2C3, (4) AYGC.3B2, (5) A2XYGC.2A2, (6) A2XYGC.2B1, (7) A2XYGC.2C1, (8) A2XYGC.3B1, (9) A2XYGC.3B3, and (10) A2XYGC.3B8, and cell lines (11) GC4 and (12) NIH/3T3. (*) DNA size marker, 1-kb ladder. When more DNA (15 μg/lane) was applied, more EYFP-probed bands can be discerned (not shown). The Cre probe hybridizes to the vector-genomic DNA junction.

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Retrovirology

ISSN: 1742-4690

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